Gene description for MUC4 |
Gene name |
mucin 4, cell surface associated |
Gene symbol |
MUC4 |
Other names/aliases |
ASGP HSA276359 MUC-4 |
Species |
Homo sapiens |
Database cross references - MUC4 |
ExoCarta |
ExoCarta_4585 |
Entrez Gene |
4585 |
HGNC |
7514 |
MIM |
158372 |
UniProt |
Q99102
|
MUC4 identified in exosomes derived from the following tissue/cell type |
Hepatocytes
|
26054723
|
Saliva
|
19199708
|
Squamous carcinoma cells
|
20124223
|
Tracheobronchial cells
|
19190083
|
Gene ontology annotations for MUC4 |
|
Experiment description of studies that identified MUC4 in exosomes |
1 |
Experiment ID |
237 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101|Alix|HSC70|GAPDH
|
EV Cytosolic markers |
✘
|
EV Membrane markers |
✔
HSP90B1
|
EV Negative markers |
✔
qNano
|
EV Particle analysis
|
|
Identified molecule |
mRNA
|
Identification method |
RNA Sequencing
|
PubMed ID |
26054723
|
Organism |
Homo sapiens |
Experiment description |
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs |
Authors |
He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N |
Journal name |
Carcinogenesis
|
Publication year |
2015 |
Sample |
Hepatocytes |
Sample name |
MIHA |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient |
Flotation density |
1.13-1.19 g/mL
|
Molecules identified in the study |
Protein RNA |
Methods used in the study |
Western blotting Mass spectrometry RNA Sequencing |
|
|
2 |
Experiment ID |
66 |
ISEV standards |
✔
IEM
|
EV Biophysical techniques |
✔
TSG101|Alix
|
EV Cytosolic markers |
✔
CD63|CD81
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
19199708
|
Organism |
Homo sapiens |
Experiment description |
Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT). |
Authors |
Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR |
Journal name |
JPR
|
Publication year |
2009 |
Sample |
Saliva |
Sample name |
Saliva |
Isolation/purification methods |
Differential centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [LTQ] Western blotting Immunoelectron Microscopy |
|
|
3 |
Experiment ID |
191 |
ISEV standards |
✘
|
EV Biophysical techniques |
✔
Alix
|
EV Cytosolic markers |
✔
CD81|CD9
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
20124223
|
Organism |
Homo sapiens |
Experiment description |
Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes. |
Authors |
Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK. |
Journal name |
Mol Cell Proteomics
|
Publication year |
2010 |
Sample |
Squamous carcinoma cells |
Sample name |
Squamous carcinoma cell (A431) |
Isolation/purification methods |
Differential centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|
4 |
Experiment ID |
34 |
ISEV standards |
✔
EM|IEM
|
EV Biophysical techniques |
✔
TSG101
|
EV Cytosolic markers |
✔
CD63|MHCI|MHCII
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Western blotting Mass spectrometry FACS
|
PubMed ID |
19190083
|
Organism |
Homo sapiens |
Experiment description |
Characterization of exosome-like vesicles released from human tracheobronchial ciliated epithelium: a possible role in innate defense. |
Authors |
Kesimer M, Scull M, Brighton B, Demaria G, Burns K, O'Neal W, Pickles RJ, Sheehan JK |
Journal name |
FASEB
|
Publication year |
2009 |
Sample |
Tracheobronchial cells |
Sample name |
Tracheobronchial epithelial cells |
Isolation/purification methods |
Differential centrifugation Filtration Sucrose density gradient |
Flotation density |
1.16 -1.18 g/mL
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
Mass spectrometry [QTOF] Western blotting Immunoelectron Microscopy FACS |
|
|
Protein-protein interactions for MUC4 |
|
Protein Interactor |
ExoCarta ID |
Identification method |
PubMed |
Species |
No interactions are found.
|
|
Pathways in which MUC4 is involved |
|
|
|