Gene description for SPON2
Gene name spondin 2, extracellular matrix protein
Gene symbol SPON2
Other names/aliases DIL-1
DIL1
M-SPONDIN
MINDIN
Species Homo sapiens
 Database cross references - SPON2
ExoCarta ExoCarta_10417
Vesiclepedia VP_10417
Entrez Gene 10417
HGNC 11253
MIM 605918
UniProt Q9BUD6  
 SPON2 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Urine 19056867    
 Gene ontology annotations for SPON2
Molecular Function
    lipopolysaccharide binding GO:0001530 IEA
    antigen binding GO:0003823 IEA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    mast cell mediated immunity GO:0002448 IEA
    cell adhesion GO:0007155 IBA
    opsonization GO:0008228 IEA
    positive regulation of interleukin-6 production GO:0032755 IEA
    positive regulation of tumor necrosis factor production GO:0032760 IEA
    induction of bacterial agglutination GO:0043152 IEA
    innate immune response GO:0045087 IEA
    defense response to fungus GO:0050832 IEA
    defense response to virus GO:0051607 IEA
    positive regulation of macrophage cytokine production GO:0060907 IEA
    cellular response to lipopolysaccharide GO:0071222 IEA
Subcellular Localization
    extracellular matrix GO:0031012 IBA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified SPON2 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 142
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name LNCaP - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
3
Experiment ID 143
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name LNCaP - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
4
Experiment ID 144
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name C4-2 - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
5
Experiment ID 145
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|CD10
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name C4-2 - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
6
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for SPON2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KRT31 3881
Two-hybrid Homo sapiens
2 DDX58 23586
Affinity Capture-RNA Homo sapiens
3 PLEKHF2 79666
Two-hybrid Homo sapiens
View the network image/svg+xml



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