Gene description for UNC13B
Gene name unc-13 homolog B (C. elegans)
Gene symbol UNC13B
Other names/aliases MUNC13
UNC13
Unc13h2
Species Homo sapiens
 Database cross references - UNC13B
ExoCarta ExoCarta_10497
Vesiclepedia VP_10497
Entrez Gene 10497
HGNC 12566
MIM 605836
UniProt O14795  
 UNC13B identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for UNC13B
Molecular Function
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IBA
    calmodulin binding GO:0005516 ISS
    phospholipid binding GO:0005543 IEA
    syntaxin-1 binding GO:0017075 IBA
    diacylglycerol binding GO:0019992 NAS
    GTP-dependent protein binding GO:0030742 IPI
    small GTPase binding GO:0031267 NAS
Biological Process
    chemical synaptic transmission GO:0007268 ISS
    positive regulation of synaptic vesicle priming GO:0010808 ISS
    synaptic vesicle docking GO:0016081 IBA
    synaptic vesicle priming GO:0016082 IBA
    synaptic vesicle priming GO:0016082 ISS
    synaptic transmission, glutamatergic GO:0035249 IBA
    positive regulation of apoptotic process GO:0043065 IDA
    positive regulation of protein secretion GO:0050714 IDA
    acrosomal vesicle exocytosis GO:0060478 IDA
    dense core granule priming GO:0061789 IBA
    cellular response to glucose stimulus GO:0071333 IDA
    positive regulation of inhibitory postsynaptic potential GO:0097151 ISS
    neuronal dense core vesicle exocytosis GO:0099011 ISS
    presynaptic dense core vesicle exocytosis GO:0099525 IBA
Subcellular Localization
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISS
    membrane GO:0016020 IDA
    synaptic vesicle membrane GO:0030672 IBA
    neuromuscular junction GO:0031594 IBA
    presynaptic membrane GO:0042734 IBA
    terminal bouton GO:0043195 IBA
    terminal bouton GO:0043195 ISS
    presynaptic active zone GO:0048786 TAS
    presynaptic active zone cytoplasmic component GO:0098831 IBA
 Experiment description of studies that identified UNC13B in sEVs
1
Experiment ID 126
MISEV standards
Biophysical techniques
GAPDH
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for UNC13B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PDCL2  
Two-hybrid Homo sapiens
2 CALML3 810
Affinity Capture-MS Homo sapiens
3 ZNF418  
Affinity Capture-MS Homo sapiens
4 VAMP2 6844
Co-fractionation Homo sapiens
5 DOC2B  
Affinity Capture-MS Homo sapiens
6 OCLN 100506658
Proximity Label-MS Homo sapiens
7 RPH3AL 9501
Reconstituted Complex Homo sapiens
8 RIMS1  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
9 MARCKS 4082
Proximity Label-MS Homo sapiens
10 SPTBN2 6712
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
11 UBXN10  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
12 GARS 2617
Affinity Capture-MS Homo sapiens
13 CALM2 805
Affinity Capture-MS Homo sapiens
14 DIRAS2 54769
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 CALM1 801
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 CUL3 8452
Affinity Capture-MS Homo sapiens
17 SNAP25 6616
Co-fractionation Homo sapiens
18 ECT2 1894
Affinity Capture-MS Homo sapiens
19 STX1B 112755
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
20 CCDC71  
Affinity Capture-MS Homo sapiens
21 LOC338797  
Affinity Capture-MS Homo sapiens
22 WDR48 57599
Affinity Capture-MS Homo sapiens
23 MKRN2 23609
Affinity Capture-RNA Homo sapiens
24 RAB27A 5873
Affinity Capture-Western Homo sapiens
25 DIRAS1 148252
Affinity Capture-MS Homo sapiens
26 BARD1 580
Affinity Capture-MS Homo sapiens
27 PEX14 5195
Proximity Label-MS Homo sapiens
28 PTPN4  
Affinity Capture-MS Homo sapiens
29 COQ3  
Affinity Capture-MS Homo sapiens
30 CALM3 808
Affinity Capture-MS Homo sapiens
31 STX1A 6804
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
32 CCDC40  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 CTCFL  
Affinity Capture-MS Homo sapiens
34 ALS2CR11  
Affinity Capture-MS Homo sapiens
35 DOC2A  
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
36 DNAJC22  
Affinity Capture-MS Homo sapiens
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