Gene description for U2af1
Gene name U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 1
Gene symbol U2af1
Other names/aliases 2010107D16Rik
35kDa
Species Mus musculus
 Database cross references - U2af1
ExoCarta ExoCarta_108121
Vesiclepedia VP_108121
Entrez Gene 108121
UniProt Q9D883  
 U2af1 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for U2af1
Molecular Function
    RNA binding GO:0003723 IEA
    protein binding GO:0005515 IPI
    pre-mRNA 3'-splice site binding GO:0030628 IBA
    metal ion binding GO:0046872 IEA
    RS domain binding GO:0050733 IPI
Biological Process
    mRNA splicing, via spliceosome GO:0000398 IBA
    mRNA splicing, via spliceosome GO:0000398 IEA
Subcellular Localization
    nucleoplasm GO:0005654 ISO
    spliceosomal complex GO:0005681 IBA
    spliceosomal complex GO:0005681 ISO
    nuclear speck GO:0016607 IDA
    catalytic step 2 spliceosome GO:0071013 IEA
    catalytic step 2 spliceosome GO:0071013 ISO
    U2AF complex GO:0089701 IBA
    U2AF complex GO:0089701 IEA
    U2AF complex GO:0089701 ISO
 Experiment description of studies that identified U2af1 in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for U2af1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kctd13  
Affinity Capture-MS Mus musculus
2 Atxn1  
Affinity Capture-MS Mus musculus
3 Eed  
Affinity Capture-MS Mus musculus
4 Map1b 17755
Affinity Capture-Western Mus musculus
5 Otx2  
Affinity Capture-MS Mus musculus
6 Fancd2  
Affinity Capture-MS Mus musculus
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