Gene description for FGD4
Gene name FYVE, RhoGEF and PH domain containing 4
Gene symbol FGD4
Other names/aliases CMT4H
FRABP
ZFYVE6
Species Homo sapiens
 Database cross references - FGD4
ExoCarta ExoCarta_121512
Vesiclepedia VP_121512
Entrez Gene 121512
HGNC 19125
MIM 611104
UniProt Q96M96  
 FGD4 identified in sEVs derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for FGD4
Molecular Function
    actin binding GO:0003779 IEA
    guanyl-nucleotide exchange factor activity GO:0005085 IBA
    guanyl-nucleotide exchange factor activity GO:0005085 ISS
    guanyl-nucleotide exchange factor activity GO:0005085 TAS
    small GTPase binding GO:0031267 ISS
    metal ion binding GO:0046872 IEA
Biological Process
    cytoskeleton organization GO:0007010 IBA
    cytoskeleton organization GO:0007010 ISS
    regulation of cell shape GO:0008360 ISS
    actin cytoskeleton organization GO:0030036 ISS
    regulation of GTPase activity GO:0043087 ISS
    filopodium assembly GO:0046847 IBA
    filopodium assembly GO:0046847 ISS
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
Subcellular Localization
    ruffle GO:0001726 ISS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISS
    Golgi apparatus GO:0005794 ISS
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 IEA
    lamellipodium GO:0030027 ISS
    filopodium GO:0030175 IEA
 Experiment description of studies that identified FGD4 in sEVs
1
Experiment ID 211
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for FGD4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GSN 2934
Affinity Capture-MS Homo sapiens
2 PLEC 5339
Affinity Capture-MS Homo sapiens
3 C9orf16  
Two-hybrid Homo sapiens
4 CDC42 998
FRET Homo sapiens
5 ACTB 60
Proximity Label-MS Homo sapiens
6 NEFL 4747
Affinity Capture-MS Homo sapiens
7 CCDC86  
Affinity Capture-MS Homo sapiens
8 FGD3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 CUL3 8452
Affinity Capture-MS Homo sapiens
10 CAPZA2 830
Affinity Capture-MS Homo sapiens
11 DNAJC9 23234
Affinity Capture-MS Homo sapiens
12 PDIA3 2923
Affinity Capture-MS Homo sapiens
13 Myh9 17886
Affinity Capture-MS Mus musculus
14 POTEE 445582
Affinity Capture-MS Homo sapiens
15 PARK2  
Affinity Capture-MS Homo sapiens
16 KIF14 9928
Affinity Capture-MS Homo sapiens
17 PRKCA 5578
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 CTDSPL2  
Affinity Capture-MS Homo sapiens
19 LANCL1 10314
Affinity Capture-MS Homo sapiens
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