Gene description for Cask
Gene name calcium/calmodulin-dependent serine protein kinase (MAGUK family)
Gene symbol Cask
Other names/aliases DXPri1
DXRib1
LIN-2
Pals3
mLin-2
Species Mus musculus
 Database cross references - Cask
ExoCarta ExoCarta_12361
Vesiclepedia VP_12361
Entrez Gene 12361
UniProt O70589  
 Cask identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cask
Molecular Function
    protein serine/threonine kinase activity GO:0004674 ISO
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein kinase C binding GO:0005080 ISO
    signaling receptor binding GO:0005102 IBA
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IEA
    ATP binding GO:0005524 IEA
    PDZ domain binding GO:0030165 ISO
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    neurexin family protein binding GO:0042043 IDA
    neurexin family protein binding GO:0042043 ISO
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    protein-containing complex binding GO:0044877 ISO
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    negative regulation of cell-matrix adhesion GO:0001953 IEA
    negative regulation of cell-matrix adhesion GO:0001953 ISO
    chromatin remodeling GO:0006338 IEA
    regulation of transcription by RNA polymerase II GO:0006357 ISO
    protein localization GO:0008104 IBA
    negative regulation of keratinocyte proliferation GO:0010839 IEA
    negative regulation of keratinocyte proliferation GO:0010839 ISO
    positive regulation of insulin secretion GO:0032024 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 IGI
    regulation of neurotransmitter secretion GO:0046928 IBA
    establishment of localization in cell GO:0051649 IMP
    positive regulation of dendritic spine morphogenesis GO:0061003 ISO
    negative regulation of wound healing GO:0061045 IEA
    negative regulation of wound healing GO:0061045 ISO
    calcium ion import GO:0070509 IMP
    protein localization to plasma membrane GO:0072659 ISO
    positive regulation of calcium ion import GO:0090280 IDA
    negative regulation of cellular response to growth factor stimulus GO:0090288 IEA
    negative regulation of cellular response to growth factor stimulus GO:0090288 ISO
    regulation of synaptic vesicle exocytosis GO:2000300 IDA
    regulation of synaptic vesicle exocytosis GO:2000300 IMP
Subcellular Localization
    basement membrane GO:0005604 IDA
    basement membrane GO:0005604 IDA
    basement membrane GO:0005604 ISO
    basement membrane GO:0005604 ISO
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nuclear lamina GO:0005652 IEA
    nuclear lamina GO:0005652 ISO
    nucleolus GO:0005730 IEA
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    cell-cell junction GO:0005911 IBA
    cell-cell junction GO:0005911 IDA
    cell-cell junction GO:0005911 ISO
    membrane GO:0016020 IDA
    basolateral plasma membrane GO:0016323 IBA
    basolateral plasma membrane GO:0016323 IDA
    basolateral plasma membrane GO:0016323 ISO
    nuclear matrix GO:0016363 IEA
    nuclear matrix GO:0016363 ISO
    dendrite GO:0030425 ISO
    vesicle GO:0031982 IDA
    protein-containing complex GO:0032991 ISO
    presynaptic membrane GO:0042734 ISO
    neuronal cell body GO:0043025 ISO
    synapse GO:0045202 IDA
    perinuclear region of cytoplasm GO:0048471 ISO
    ciliary membrane GO:0060170 ISO
    synaptic membrane GO:0097060 ISO
    apical dendrite GO:0097440 ISO
    Schaffer collateral - CA1 synapse GO:0098685 IDA
    Schaffer collateral - CA1 synapse GO:0098685 IMP
    podocyte foot GO:0098846 ISO
 Experiment description of studies that identified Cask in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cask
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NET1 10276
Protein-peptide Homo sapiens
2 Tax1bp3  
Co-fractionation Mus musculus
3 Tnik  
Affinity Capture-MS Mus musculus
4 PARP12 64761
Two-hybrid Homo sapiens
5 Kctd13  
Affinity Capture-MS Mus musculus
6 Lin7c 22343
Affinity Capture-MS Mus musculus
Affinity Capture-Western Mus musculus
7 SF1 7536
Two-hybrid Homo sapiens
8 UTP14A 10813
Two-hybrid Homo sapiens
9 PRPF6 24148
Two-hybrid Homo sapiens
10 MAP7 9053
Two-hybrid Homo sapiens
11 Park2  
Affinity Capture-Western Mus musculus
12 Tiam2  
Co-fractionation Mus musculus
13 Ppfia2  
Affinity Capture-Western Mus musculus
14 Dlgap1  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here