Gene description for Ilf3
Gene name interleukin enhancer binding factor 3
Gene symbol Ilf3
Other names/aliases MBII-26
MPHOSPH4
NF90
NFAR
Species Mus musculus
 Database cross references - Ilf3
ExoCarta ExoCarta_16201
Vesiclepedia VP_16201
Entrez Gene 16201
UniProt Q9Z1X4  
 Ilf3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ilf3
Molecular Function
    virus receptor activity GO:0001618 IEA
    virus receptor activity GO:0001618 ISO
    DNA binding GO:0003677 ISO
    DNA binding GO:0003677 TAS
    double-stranded RNA binding GO:0003725 IBA
    double-stranded RNA binding GO:0003725 IEA
    double-stranded RNA binding GO:0003725 ISO
    single-stranded RNA binding GO:0003727 IBA
    enzyme binding GO:0019899 ISO
    mRNA 3'-UTR AU-rich region binding GO:0035925 ISS
Biological Process
    protein phosphorylation GO:0006468 IDA
    protein phosphorylation GO:0006468 ISO
    protein methylation GO:0006479 ISO
    negative regulation of translation GO:0017148 IMP
    negative regulation of viral genome replication GO:0045071 IMP
    negative regulation of DNA-templated transcription GO:0045892 IEA
    negative regulation of DNA-templated transcription GO:0045892 ISO
    positive regulation of DNA-templated transcription GO:0045893 IEA
    positive regulation of DNA-templated transcription GO:0045893 ISO
    symbiont entry into host cell GO:0046718 IEA
    defense response to virus GO:0051607 IEA
    spliceosome-depend formation of circular RNA GO:0160091 ISS
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    nucleolus GO:0005730 IEA
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 IEA
    mitochondrion GO:0005739 ISO
    ribonucleoprotein complex GO:1990904 ISO
    ribonucleoprotein complex GO:1990904 ISS
 Experiment description of studies that identified Ilf3 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ilf3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fxr1 14359
Affinity Capture-MS Mus musculus
2 Elf5  
Affinity Capture-MS Mus musculus
3 Ncbp1 433702
Co-fractionation Mus musculus
4 Rpl13a 22121
Co-fractionation Mus musculus
5 Atxn2 20239
Co-fractionation Mus musculus
6 Stau1  
Co-fractionation Mus musculus
7 Pou5f1  
Affinity Capture-MS Mus musculus
8 G3bp2  
Two-hybrid Mus musculus
9 Fmr1  
Co-fractionation Mus musculus
10 Kctd13  
Affinity Capture-MS Mus musculus
11 Ncl 17975
Co-fractionation Mus musculus
12 Ddx17 67040
Co-fractionation Mus musculus
13 Tmem173  
Proximity Label-MS Mus musculus
14 Fzr1  
Affinity Capture-MS Mus musculus
15 Rps9 76846
Co-fractionation Mus musculus
16 Nanog  
Affinity Capture-MS Mus musculus
17 Zbtb7b  
Affinity Capture-MS Mus musculus
18 DHX9 1660
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
19 Eed  
Affinity Capture-MS Mus musculus
20 Sox17  
Affinity Capture-MS Mus musculus
21 Ube2i  
Two-hybrid Mus musculus
22 ADARB1 104
Two-hybrid Homo sapiens
23 Cpsf6  
Two-hybrid Mus musculus
24 PRKRA 8575
Two-hybrid Homo sapiens
25 Fancd2  
Affinity Capture-MS Mus musculus
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