Gene description for Mical1
Gene name microtubule associated monooxygenase, calponin and LIM domain containing 1
Gene symbol Mical1
Other names/aliases MICAL
MICAL-1
Nical
Species Mus musculus
 Database cross references - Mical1
ExoCarta ExoCarta_171580
Vesiclepedia VP_171580
Entrez Gene 171580
UniProt Q8VDP3  
 Mical1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Mical1
Molecular Function
    actin binding GO:0003779 IDA
    actin binding GO:0003779 ISO
    actin binding GO:0003779 ISS
    monooxygenase activity GO:0004497 IMP
    monooxygenase activity GO:0004497 ISO
    protein binding GO:0005515 IPI
    NAD(P)H oxidase H2O2-forming activity GO:0016174 ISO
    oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen GO:0016709 IDA
    oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen GO:0016709 ISO
    oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen GO:0016709 ISS
    SH3 domain binding GO:0017124 ISO
    SH3 domain binding GO:0017124 ISS
    protein kinase binding GO:0019901 IDA
    small GTPase binding GO:0031267 IPI
    metal ion binding GO:0046872 IEA
    FAD binding GO:0071949 IDA
    FAD binding GO:0071949 ISO
    FAD binding GO:0071949 ISS
    NADPH oxidase H202-forming activity GO:0106294 IEA
    F-actin monooxygenase activity GO:0120501 IEA
Biological Process
    negative regulation of protein phosphorylation GO:0001933 IMP
    sulfur oxidation GO:0019417 IDA
    actin filament depolymerization GO:0030042 IDA
    actin filament depolymerization GO:0030042 ISO
    actin filament depolymerization GO:0030042 ISS
    negative regulation of apoptotic process GO:0043066 IMP
    regulation of regulated secretory pathway GO:1903305 IMP
    negative regulation of cysteine-type endopeptidase activity GO:2000117 IMP
Subcellular Localization
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    actin filament GO:0005884 IEA
    actin filament GO:0005884 ISO
    endosome membrane GO:0010008 ISO
    endosome membrane GO:0010008 ISS
    midbody GO:0030496 IEA
    midbody GO:0030496 ISO
    intercellular bridge GO:0045171 IEA
    intercellular bridge GO:0045171 ISO
    hippocampal mossy fiber expansion GO:1990026 IMP
 Experiment description of studies that identified Mical1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Mical1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rab10 19325
Co-fractionation Mus musculus
2 Atg16l1  
Affinity Capture-MS Mus musculus
3 Kctd13  
Affinity Capture-MS Mus musculus
4 Tmem173  
Proximity Label-MS Mus musculus
5 Qrsl1  
Co-fractionation Mus musculus
View the network image/svg+xml
 Pathways in which Mical1 is involved
No pathways found





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