Experiment description of studies that identified Met in sEVs

1 Experiment ID 520 MISEV standards ✔ EM Biophysical techniques ✔ Tsg101|Cd9 Enriched markers ✔ Golga2 Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 34112803     Organism Mus musculus Experiment description Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution Authors "Lima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A. " Journal name Nat Commun Publication year 2021 Sample Breast cancer cells Sample name EO771 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Centrifugal ultrafiltration Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

2 Experiment ID 521 MISEV standards ✔ EM Biophysical techniques ✔ Tsg101|Cd9 Enriched markers ✔ Golga2 Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 34112803     Organism Mus musculus Experiment description Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution Authors "Lima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A. " Journal name Nat Commun Publication year 2021 Sample Breast cancer cells Sample name PyMT Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography Centrifugal ultrafiltration Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectometry

3 Experiment ID 1101 MISEV standards ✔ EM Biophysical techniques ✔ Cd81|Cd9|Tfrc|Gapdh|Lamp1 Enriched markers ✔ GOLGA2|CANX Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 34957415     Organism Mus musculus Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H." Journal name Nat Cancer Publication year 2021 Sample Melanoma cells Sample name B16-F1 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

4 Experiment ID 1102 MISEV standards ✔ EM Biophysical techniques ✔ Cd81|Cd9|Tfrc|Gapdh|Lamp1 Enriched markers ✔ GOLGA2|CANX Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 34957415     Organism Mus musculus Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H." Journal name Nat Cancer Publication year 2021 Sample Melanoma cells Sample name B16-F10 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry Flow cytometry

5 Experiment ID 1103 MISEV standards ✔ EM Biophysical techniques ✔ Cd81|Cd9|Tfrc|Gapdh|Lamp1 Enriched markers ✔ GOLGA2|CANX Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 34957415     Organism Mus musculus Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H." Journal name Nat Cancer Publication year 2021 Sample Melanoma cells Sample name B16-F1R2 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry Flow cytometry

Protein-protein interactions for Met

Protein Interactor ExoCarta ID Identification method PubMed Species 1 Grb2 14784 Reconstituted Complex Mus musculus Affinity Capture-Western Mus musculus 2 Gab1   Phenotypic Enhancement Mus musculus Affinity Capture-Western Mus musculus 3 Muc20   Affinity Capture-Western Mus musculus Affinity Capture-Western Mus musculus View the network

Pathways in which Met is involved

No pathways found