Gene description for AGL
Gene name amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase
Gene symbol AGL
Other names/aliases GDE
Species Homo sapiens
 Database cross references - AGL
ExoCarta ExoCarta_178
Vesiclepedia VP_178
Entrez Gene 178
HGNC 321
MIM 610860
UniProt P35573  
 AGL identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for AGL
Molecular Function
    glycogen debranching enzyme activity GO:0004133 IEA
    4-alpha-glucanotransferase activity GO:0004134 EXP
    4-alpha-glucanotransferase activity GO:0004134 IBA
    amylo-alpha-1,6-glucosidase activity GO:0004135 EXP
    amylo-alpha-1,6-glucosidase activity GO:0004135 IBA
    protein binding GO:0005515 IPI
    polysaccharide binding GO:0030247 IEA
    polyubiquitin modification-dependent protein binding GO:0031593 IEA
Biological Process
    glycogen biosynthetic process GO:0005978 IEA
    glycogen catabolic process GO:0005980 IBA
    glycogen catabolic process GO:0005980 TAS
    response to nutrient GO:0007584 IEA
    response to glucocorticoid GO:0051384 IEA
Subcellular Localization
    extracellular region GO:0005576 TAS
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    inclusion body GO:0016234 IEA
    sarcoplasmic reticulum GO:0016529 IEA
    secretory granule lumen GO:0034774 TAS
    isoamylase complex GO:0043033 TAS
    ficolin-1-rich granule lumen GO:1904813 TAS
 Experiment description of studies that identified AGL in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for AGL
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 USP5 8078
Co-fractionation Homo sapiens
2 FKBP1B  
Co-fractionation Homo sapiens
3 CAT 847
Co-fractionation Homo sapiens
4 ASB3 51130
Affinity Capture-MS Homo sapiens
5 AHSA1 10598
Affinity Capture-MS Homo sapiens
6 LGR4 55366
Affinity Capture-MS Homo sapiens
7 YTHDF2 51441
Co-fractionation Homo sapiens
8 MAN2B1 4125
Co-fractionation Homo sapiens
9 SDHB 6390
Co-fractionation Homo sapiens
10 ASB15 142685
Affinity Capture-MS Homo sapiens
11 PRNP 5621
Affinity Capture-MS Homo sapiens
12 GCLC 2729
Co-fractionation Homo sapiens
13 PKM 5315
Co-fractionation Homo sapiens
14 KIAA1715 80856
Affinity Capture-MS Homo sapiens
15 GARS 2617
Co-fractionation Homo sapiens
16 FOXA2  
Affinity Capture-MS Homo sapiens
17 ASPSCR1 79058
Co-fractionation Homo sapiens
18 GRHPR 9380
Co-fractionation Homo sapiens
19 PRKAA2 5563
Affinity Capture-MS Homo sapiens
20 GYG2  
Affinity Capture-MS Homo sapiens
21 CUL5 8065
Affinity Capture-MS Homo sapiens
22 CUL3 8452
Affinity Capture-MS Homo sapiens
23 KHK 3795
Co-fractionation Homo sapiens
24 GCK  
Co-fractionation Homo sapiens
25 RNF126  
Affinity Capture-MS Homo sapiens
26 STBD1 8987
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 CUL4B 8450
Affinity Capture-MS Homo sapiens
28 RNPEP 6051
Co-fractionation Homo sapiens
29 FKBP1A 2280
Co-fractionation Homo sapiens
30 CUL1 8454
Affinity Capture-MS Homo sapiens
31 XYLB 9942
Co-fractionation Homo sapiens
32 NXF1 10482
Affinity Capture-RNA Homo sapiens
33 APEX1 328
Affinity Capture-RNA Homo sapiens
34 MCFD2 90411
Co-fractionation Homo sapiens
35 GLO1 2739
Co-fractionation Homo sapiens
36 ARIH2 10425
Affinity Capture-MS Homo sapiens
37 DUSP12 11266
Co-fractionation Homo sapiens
38 SERPINE2 5270
Co-fractionation Homo sapiens
39 DCUN1D1 54165
Affinity Capture-MS Homo sapiens
40 MOV10 4343
Affinity Capture-RNA Homo sapiens
41 VCL 7414
Cross-Linking-MS (XL-MS) Homo sapiens
42 Ppp1r3b  
Affinity Capture-MS Mus musculus
43 RAN 5901
Co-fractionation Homo sapiens
44 NTRK1 4914
Affinity Capture-MS Homo sapiens
45 PYGB 5834
Affinity Capture-MS Homo sapiens
46 NHLRC1  
Affinity Capture-Western Homo sapiens
47 CARKD 55739
Co-fractionation Homo sapiens
48 IGF2BP2 10644
Co-fractionation Homo sapiens
49 COPS5 10987
Affinity Capture-MS Homo sapiens
50 SULT1E1 6783
Co-fractionation Homo sapiens
51 ADSS 159
Co-fractionation Homo sapiens
52 C9orf72  
Affinity Capture-MS Homo sapiens
53 PSMB6 5694
Co-fractionation Homo sapiens
54 TKFC 26007
Co-fractionation Homo sapiens
55 PRKAB2 5565
Affinity Capture-MS Homo sapiens
56 CUL2 8453
Affinity Capture-MS Homo sapiens
57 DERA 51071
Co-fractionation Homo sapiens
58 SPATA20  
Co-fractionation Homo sapiens
59 PUS1 80324
Co-fractionation Homo sapiens
60 CDK9 1025
Co-fractionation Homo sapiens
61 RPA3 6119
Proximity Label-MS Homo sapiens
62 BCAT1 586
Co-fractionation Homo sapiens
63 RASSF5 83593
Affinity Capture-MS Homo sapiens
64 TOM1L2 146691
Co-fractionation Homo sapiens
65 VPS25 84313
Co-fractionation Homo sapiens
66 SP100 6672
Co-fractionation Homo sapiens
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