Gene description for Ppp1r12a
Gene name protein phosphatase 1, regulatory (inhibitor) subunit 12A
Gene symbol Ppp1r12a
Other names/aliases 1200015F06Rik
5730577I22Rik
AA792106
AV099298
D10Ertd625e
Mypt1
Species Mus musculus
 Database cross references - Ppp1r12a
ExoCarta ExoCarta_17931
Vesiclepedia VP_17931
Entrez Gene 17931
UniProt Q9DBR7  
 Ppp1r12a identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Ppp1r12a
Molecular Function
    phosphoprotein phosphatase activity GO:0004721 ISO
    enzyme inhibitor activity GO:0004857 IBA
    enzyme inhibitor activity GO:0004857 ISO
    enzyme inhibitor activity GO:0004857 ISS
    protein binding GO:0005515 IPI
    myosin phosphatase regulator activity GO:0017020 IBA
    phosphatase regulator activity GO:0019208 ISO
    phosphatase regulator activity GO:0019208 ISS
    protein kinase binding GO:0019901 IEA
    protein kinase binding GO:0019901 ISO
    14-3-3 protein binding GO:0071889 ISO
    14-3-3 protein binding GO:0071889 ISS
Biological Process
    mitotic cell cycle GO:0000278 ISO
    mitotic cell cycle GO:0000278 ISS
    protein dephosphorylation GO:0006470 IMP
    protein dephosphorylation GO:0006470 ISO
    centrosome cycle GO:0007098 ISO
    centrosome cycle GO:0007098 ISS
    signal transduction GO:0007165 IEA
    regulation of cell adhesion GO:0030155 ISO
    regulation of cell adhesion GO:0030155 ISS
    negative regulation of catalytic activity GO:0043086 ISO
    negative regulation of catalytic activity GO:0043086 ISS
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    positive regulation of transcription by RNA polymerase II GO:0045944 ISS
    regulation of nucleocytoplasmic transport GO:0046822 IMP
    neuron projection morphogenesis GO:0048812 IBA
    cellular response to xenobiotic stimulus GO:0071466 IDA
Subcellular Localization
    kinetochore GO:0000776 ISO
    kinetochore GO:0000776 ISS
    stress fiber GO:0001725 IEA
    nucleolus GO:0005730 IEA
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 IBA
    centrosome GO:0005813 ISO
    centrosome GO:0005813 ISS
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    actin cytoskeleton GO:0015629 ISO
    Z disc GO:0030018 IBA
    Z disc GO:0030018 IDA
    A band GO:0031672 IBA
    A band GO:0031672 IDA
    contractile muscle fiber GO:0043292 ISO
    PTW/PP1 phosphatase complex GO:0072357 ISO
    PTW/PP1 phosphatase complex GO:0072357 ISS
 Experiment description of studies that identified Ppp1r12a in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Ppp1r12a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kctd13  
Affinity Capture-MS Mus musculus
2 Mycbp2 105689
Affinity Capture-MS Mus musculus
3 Pus1  
Co-fractionation Mus musculus
4 Atg16l1  
Affinity Capture-MS Mus musculus
5 Chaf1a  
Affinity Capture-MS Mus musculus
6 Ash1l  
Co-fractionation Mus musculus
7 Rhoa 11848
Co-fractionation Mus musculus
View the network image/svg+xml



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