Gene description for Ppp1ca
Gene name protein phosphatase 1, catalytic subunit, alpha isoform
Gene symbol Ppp1ca
Other names/aliases Ppp1c
dism2
Species Mus musculus
 Database cross references - Ppp1ca
ExoCarta ExoCarta_19045
Vesiclepedia VP_19045
Entrez Gene 19045
UniProt P62137  
 Ppp1ca identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ppp1ca
Molecular Function
    phosphoprotein phosphatase activity GO:0004721 IDA
    phosphoprotein phosphatase activity GO:0004721 ISO
    protein serine/threonine phosphatase activity GO:0004722 IBA
    protein serine/threonine phosphatase activity GO:0004722 IDA
    protein serine/threonine phosphatase activity GO:0004722 ISO
    protein serine/threonine phosphatase activity GO:0004722 ISS
    protein serine/threonine phosphatase activity GO:0004722 TAS
    iron ion binding GO:0005506 ISS
    protein binding GO:0005515 IPI
    protein phosphatase 1 binding GO:0008157 IEA
    protein phosphatase 1 binding GO:0008157 ISO
    phosphatase activity GO:0016791 IDA
    myosin phosphatase activity GO:0017018 IEA
    calmodulin-dependent protein phosphatase activity GO:0033192 IEA
    ribonucleoprotein complex binding GO:0043021 IEA
    ribonucleoprotein complex binding GO:0043021 ISO
    protein-containing complex binding GO:0044877 ISO
    transition metal ion binding GO:0046914 ISS
    cadherin binding involved in cell-cell adhesion GO:0098641 IEA
    cadherin binding involved in cell-cell adhesion GO:0098641 ISO
    histone H2AXS140 phosphatase activity GO:0140791 IEA
    RNA polymerase II CTD heptapeptide repeat Y1 phosphatase activity GO:0180004 IEA
    RNA polymerase II CTD heptapeptide repeat T4 phosphatase activity GO:0180005 IEA
    RNA polymerase II CTD heptapeptide repeat S2 phosphatase activity GO:0180006 IEA
    RNA polymerase II CTD heptapeptide repeat S5 phosphatase activity GO:0180007 IEA
    RNA polymerase II CTD heptapeptide repeat S7 phosphatase activity GO:0180008 IEA
    MAP kinase serine/threonine phosphatase activity GO:1990439 IEA
Biological Process
    glycogen metabolic process GO:0005977 TAS
    regulation of glycogen biosynthetic process GO:0005979 ISO
    regulation of glycogen catabolic process GO:0005981 IEA
    regulation of glycogen catabolic process GO:0005981 ISO
    chromatin remodeling GO:0006338 IEA
    regulation of transcription by RNA polymerase II GO:0006357 IEA
    regulation of translation GO:0006417 TAS
    protein dephosphorylation GO:0006470 IDA
    protein dephosphorylation GO:0006470 IDA
    protein dephosphorylation GO:0006470 ISO
    protein dephosphorylation GO:0006470 ISO
    protein dephosphorylation GO:0006470 TAS
    response to lead ion GO:0010288 IEA
    response to lead ion GO:0010288 ISO
    dephosphorylation GO:0016311 ISO
    lung development GO:0030324 IMP
    circadian regulation of gene expression GO:0032922 IDA
    regulation of circadian rhythm GO:0042752 IDA
    regulation of circadian rhythm GO:0042752 IMP
    regulation of circadian rhythm GO:0042752 ISO
    entrainment of circadian clock by photoperiod GO:0043153 IMP
    telomere maintenance in response to DNA damage GO:0043247 IEA
    telomere maintenance in response to DNA damage GO:0043247 ISO
    positive regulation of glycogen biosynthetic process GO:0045725 IMP
    branching morphogenesis of an epithelial tube GO:0048754 IMP
    cell division GO:0051301 IEA
    cell-cell adhesion GO:0098609 IEA
    positive regulation of extrinsic apoptotic signaling pathway in absence of ligand GO:2001241 IMP
Subcellular Localization
    protein phosphatase type 1 complex GO:0000164 IMP
    protein phosphatase type 1 complex GO:0000164 ISO
    chromosome, telomeric region GO:0000781 IEA
    chromosome, telomeric region GO:0000781 ISO
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 ISO
    nucleolus GO:0005730 IEA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    adherens junction GO:0005912 IEA
    adherens junction GO:0005912 ISO
    glycogen granule GO:0042587 IEA
    glycogen granule GO:0042587 ISO
    neuronal cell body GO:0043025 ISO
    dendritic spine GO:0043197 IEA
    dendritic spine GO:0043197 ISO
    perikaryon GO:0043204 IEA
    perikaryon GO:0043204 ISO
    PTW/PP1 phosphatase complex GO:0072357 ISO
    PTW/PP1 phosphatase complex GO:0072357 ISS
    presynapse GO:0098793 IEA
    presynapse GO:0098793 ISO
    postsynapse GO:0098794 ISO
    glutamatergic synapse GO:0098978 IEA
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Ppp1ca in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ppp1ca
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Kctd13  
Affinity Capture-MS Mus musculus
2 Tnik  
Affinity Capture-MS Mus musculus
3 Cdc34  
Affinity Capture-Western Mus musculus
4 SNCA 6622
Affinity Capture-MS Homo sapiens
5 Wdr82  
Co-fractionation Mus musculus
6 Apaf1 11783
Affinity Capture-Western Mus musculus
7 Mycbp2 105689
Affinity Capture-MS Mus musculus
8 Ccnb1  
Affinity Capture-Western Mus musculus
9 Atr  
Affinity Capture-Western Mus musculus
10 Snrpd1 20641
Co-fractionation Mus musculus
11 Bcl2  
Affinity Capture-Western Mus musculus
12 Eif2a  
Affinity Capture-Western Mus musculus
13 Pou5f1  
Affinity Capture-MS Mus musculus
14 Lbr  
Affinity Capture-Western Mus musculus
15 Ccna1  
Affinity Capture-Western Mus musculus
16 Dlgap1  
Affinity Capture-MS Mus musculus
17 Skp1a 21402
Affinity Capture-Western Mus musculus
18 Nek2  
Affinity Capture-Western Mus musculus
19 Ppp2r4 110854
Affinity Capture-Western Mus musculus
20 Fmr1  
Affinity Capture-MS Mus musculus
21 Cebpa  
Affinity Capture-MS Mus musculus
22 Dlg4  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
23 Snrpd2 107686
Co-fractionation Mus musculus
24 Id2  
Affinity Capture-Western Mus musculus
25 Ccnd3  
Affinity Capture-Western Mus musculus
26 Chaf1a  
Affinity Capture-MS Mus musculus
27 Ptk2  
Affinity Capture-Western Mus musculus
28 Ccne1  
Affinity Capture-Western Mus musculus
29 Herc2  
Affinity Capture-MS Mus musculus
30 TGFBR1 7046
Affinity Capture-Luminescence Homo sapiens
31 Sfpq 71514
Co-fractionation Mus musculus
32 Cdkn2a  
Affinity Capture-Western Mus musculus
33 Agap2  
Affinity Capture-MS Mus musculus
34 Atm  
Affinity Capture-Western Mus musculus
35 Tsc1  
Affinity Capture-Western Mus musculus
36 Hspa1b 15511
Affinity Capture-Western Mus musculus
37 Pcna 18538
Affinity Capture-Western Mus musculus
38 SH3KBP1 30011
Affinity Capture-MS Homo sapiens
39 Eed  
Affinity Capture-MS Mus musculus
40 Traf3  
Proximity Label-MS Mus musculus
41 Mapt  
Affinity Capture-Western Mus musculus
42 Atg16l1  
Affinity Capture-MS Mus musculus
43 Wbp11  
Reconstituted Complex Mus musculus
44 Rpap3  
Co-fractionation Mus musculus
45 Max  
Affinity Capture-Western Mus musculus
46 Apc  
Biochemical Activity Mus musculus
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