Gene description for Ppp3ca
Gene name protein phosphatase 3, catalytic subunit, alpha isoform
Gene symbol Ppp3ca
Other names/aliases 2900074D19Rik
CN
Caln
Calna
CnA
Species Mus musculus
 Database cross references - Ppp3ca
ExoCarta ExoCarta_19055
Vesiclepedia VP_19055
Entrez Gene 19055
UniProt P63328  
 Ppp3ca identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ppp3ca
Molecular Function
    phosphoprotein phosphatase activity GO:0004721 IDA
    phosphoprotein phosphatase activity GO:0004721 ISO
    protein serine/threonine phosphatase activity GO:0004722 ISO
    calcium-dependent protein serine/threonine phosphatase activity GO:0004723 ISO
    calcium-dependent protein serine/threonine phosphatase activity GO:0004723 TAS
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IBA
    calmodulin binding GO:0005516 IDA
    calmodulin binding GO:0005516 ISO
    cyclosporin A binding GO:0016018 ISO
    myosin phosphatase activity GO:0017018 IEA
    enzyme binding GO:0019899 ISO
    calmodulin-dependent protein phosphatase activity GO:0033192 IBA
    calmodulin-dependent protein phosphatase activity GO:0033192 IDA
    calmodulin-dependent protein phosphatase activity GO:0033192 ISO
    calmodulin-dependent protein phosphatase activity GO:0033192 ISS
    protein-containing complex binding GO:0044877 IEA
    protein-containing complex binding GO:0044877 ISO
    metal ion binding GO:0046872 IEA
    protein dimerization activity GO:0046983 IEA
    protein dimerization activity GO:0046983 ISO
    ATPase binding GO:0051117 IEA
    ATPase binding GO:0051117 ISO
    histone H2AXS140 phosphatase activity GO:0140791 IEA
    RNA polymerase II CTD heptapeptide repeat Y1 phosphatase activity GO:0180004 IEA
    RNA polymerase II CTD heptapeptide repeat T4 phosphatase activity GO:0180005 IEA
    RNA polymerase II CTD heptapeptide repeat S2 phosphatase activity GO:0180006 IEA
    RNA polymerase II CTD heptapeptide repeat S5 phosphatase activity GO:0180007 IEA
    RNA polymerase II CTD heptapeptide repeat S7 phosphatase activity GO:0180008 IEA
    MAP kinase serine/threonine phosphatase activity GO:1990439 IEA
Biological Process
    G1/S transition of mitotic cell cycle GO:0000082 IMP
    response to amphetamine GO:0001975 IEA
    chromatin remodeling GO:0006338 IEA
    protein dephosphorylation GO:0006470 IDA
    protein dephosphorylation GO:0006470 IMP
    protein dephosphorylation GO:0006470 ISO
    protein import into nucleus GO:0006606 IDA
    protein import into nucleus GO:0006606 IMP
    protein import into nucleus GO:0006606 ISO
    calcium ion transport GO:0006816 IMP
    epidermis development GO:0008544 IMP
    positive regulation of cardiac muscle hypertrophy GO:0010613 IGI
    positive regulation of gene expression GO:0010628 IDA
    negative regulation of gene expression GO:0010629 IDA
    transition between fast and slow fiber GO:0014883 IDA
    cardiac muscle hypertrophy in response to stress GO:0014898 IGI
    dephosphorylation GO:0016311 IDA
    calcium-mediated signaling GO:0019722 IGI
    negative regulation of signaling GO:0023057 IMP
    keratinocyte differentiation GO:0030216 IMP
    positive regulation of cell migration GO:0030335 IEA
    positive regulation of cell migration GO:0030335 ISO
    calcineurin-NFAT signaling cascade GO:0033173 IBA
    calcineurin-NFAT signaling cascade GO:0033173 IDA
    calcineurin-NFAT signaling cascade GO:0033173 IGI
    calcineurin-NFAT signaling cascade GO:0033173 IMP
    calcineurin-NFAT signaling cascade GO:0033173 ISO
    multicellular organismal response to stress GO:0033555 IDA
    negative regulation of chromatin binding GO:0035562 IMP
    positive regulation of activated T cell proliferation GO:0042104 IMP
    positive regulation of osteoblast differentiation GO:0045669 IMP
    positive regulation of osteoclast differentiation GO:0045672 IMP
    positive regulation of cell adhesion GO:0045785 ISO
    positive regulation of endocytosis GO:0045807 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IGI
    positive regulation of transcription by RNA polymerase II GO:0045944 IGI
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    negative regulation of insulin secretion GO:0046676 IEA
    negative regulation of insulin secretion GO:0046676 ISO
    positive regulation of saliva secretion GO:0046878 IMP
    skeletal muscle fiber development GO:0048741 IMP
    skeletal muscle fiber development GO:0048741 IMP
    dendrite morphogenesis GO:0048813 IMP
    negative regulation of dendrite morphogenesis GO:0050774 IMP
    modulation of chemical synaptic transmission GO:0050804 IMP
    modulation of chemical synaptic transmission GO:0050804 IMP
    positive regulation of DNA-binding transcription factor activity GO:0051091 IDA
    response to calcium ion GO:0051592 ISO
    response to calcium ion GO:0051592 ISS
    excitatory postsynaptic potential GO:0060079 IGI
    regulation of cell proliferation involved in kidney morphogenesis GO:0061006 IMP
    peptidyl-serine dephosphorylation GO:0070262 ISO
    positive regulation of calcineurin-NFAT signaling cascade GO:0070886 NAS
    cellular response to glucose stimulus GO:0071333 IEA
    cellular response to glucose stimulus GO:0071333 ISO
    positive regulation of glomerulus development GO:0090193 IMP
    renal filtration GO:0097205 IMP
    calcineurin-mediated signaling GO:0097720 ISO
    postsynaptic modulation of chemical synaptic transmission GO:0099170 IDA
    postsynaptic modulation of chemical synaptic transmission GO:0099170 IMP
    negative regulation of angiotensin-activated signaling pathway GO:0110062 IEA
    negative regulation of angiotensin-activated signaling pathway GO:0110062 ISO
    positive regulation of cardiac muscle hypertrophy in response to stress GO:1903244 IDA
    positive regulation of connective tissue replacement GO:1905205 IGI
    positive regulation of calcium ion import across plasma membrane GO:1905665 NAS
    negative regulation of calcium ion import across plasma membrane GO:1905949 NAS
Subcellular Localization
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 NAS
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 ISO
    calcineurin complex GO:0005955 IBA
    calcineurin complex GO:0005955 IDA
    calcineurin complex GO:0005955 ISO
    calcineurin complex GO:0005955 NAS
    protein serine/threonine phosphatase complex GO:0008287 NAS
    cytoplasmic side of plasma membrane GO:0009898 IEA
    cytoplasmic side of plasma membrane GO:0009898 ISO
    Z disc GO:0030018 IDA
    slit diaphragm GO:0036057 IEA
    slit diaphragm GO:0036057 ISO
    sarcolemma GO:0042383 IEA
    dendritic spine GO:0043197 IEA
    dendritic spine GO:0043197 ISO
    synapse GO:0045202 ISO
    Schaffer collateral - CA1 synapse GO:0098685 IDA
    Schaffer collateral - CA1 synapse GO:0098685 IMP
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
 Experiment description of studies that identified Ppp3ca in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ppp3ca
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Atg5  
Affinity Capture-RNA Mus musculus
2 Tsn 22099
Co-fractionation Mus musculus
3 Lnx1  
Affinity Capture-MS Mus musculus
4 Tsc1  
Affinity Capture-MS Mus musculus
5 Tmem173  
Proximity Label-MS Mus musculus
6 Grin2b  
Affinity Capture-Western Mus musculus
7 Homer1  
Affinity Capture-MS Mus musculus
8 Kctd13  
Affinity Capture-MS Mus musculus
9 Rcan1  
Co-fractionation Mus musculus
10 Agap2  
Affinity Capture-MS Mus musculus
11 Rcan2  
Co-fractionation Mus musculus
12 Dlg4  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
13 Lnx2  
Affinity Capture-MS Mus musculus
14 Mapt  
Affinity Capture-MS Mus musculus
15 Shank3  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
16 Bcl2  
Affinity Capture-Western Mus musculus
17 Inadl  
Affinity Capture-MS Mus musculus
18 Grin1  
Affinity Capture-Western Mus musculus
Affinity Capture-MS Mus musculus
19 Ywhaz 22631
Affinity Capture-MS Mus musculus
20 MTNR1B  
Reconstituted Complex Homo sapiens
21 Dlgap1  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here