Gene description for Dad1
Gene name defender against cell death 1
Gene symbol Dad1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Dad1
ExoCarta ExoCarta_192275
Vesiclepedia VP_192275
Entrez Gene 192275
UniProt P61805  
 Dad1 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
 Gene ontology annotations for Dad1
Molecular Function
    enzyme activator activity GO:0008047 IEA
    enzyme activator activity GO:0008047 ISO
Biological Process
    blastocyst development GO:0001824 IEA
    blastocyst development GO:0001824 ISO
    protein glycosylation GO:0006486 ISO
    protein glycosylation GO:0006486 ISS
    protein N-linked glycosylation GO:0006487 IBA
    apoptotic process GO:0006915 IEA
    apoptotic process GO:0006915 ISO
    response to nutrient GO:0007584 IEP
    response to xenobiotic stimulus GO:0009410 IEP
    protein N-linked glycosylation via asparagine GO:0018279 IEA
    protein N-linked glycosylation via asparagine GO:0018279 ISO
    regulation of protein stability GO:0031647 IEA
    regulation of protein stability GO:0031647 ISO
    negative regulation of apoptotic process GO:0043066 IEA
    negative regulation of apoptotic process GO:0043066 ISO
Subcellular Localization
    oligosaccharyltransferase complex GO:0008250 IBA
    oligosaccharyltransferase complex GO:0008250 IEA
    oligosaccharyltransferase complex GO:0008250 ISO
    oligosaccharyltransferase complex GO:0008250 ISS
 Experiment description of studies that identified Dad1 in sEVs
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for Dad1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Dad1 is involved
No pathways found





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