Gene description for F9
Gene name coagulation factor IX
Gene symbol F9
Other names/aliases FIX
HEMB
P19
PTC
THPH8
Species Homo sapiens
 Database cross references - F9
ExoCarta ExoCarta_2158
Vesiclepedia VP_2158
Entrez Gene 2158
HGNC 3551
MIM 300746
UniProt P00740  
 F9 identified in sEVs derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
Prostate cancer cells 25844599    
 Gene ontology annotations for F9
Molecular Function
    endopeptidase activity GO:0004175 IDA
    serine-type endopeptidase activity GO:0004252 IBA
    serine-type endopeptidase activity GO:0004252 NAS
    calcium ion binding GO:0005509 EXP
    calcium ion binding GO:0005509 IDA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 EXP
Biological Process
    proteolysis GO:0006508 IDA
    blood coagulation GO:0007596 IBA
    blood coagulation GO:0007596 IDA
    blood coagulation GO:0007596 IMP
    zymogen activation GO:0031638 IBA
    zymogen activation GO:0031638 IDA
Subcellular Localization
    extracellular region GO:0005576 NAS
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 IDA
    endoplasmic reticulum lumen GO:0005788 TAS
    Golgi lumen GO:0005796 TAS
    plasma membrane GO:0005886 TAS
    collagen-containing extracellular matrix GO:0062023 HDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified F9 in sEVs
1
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
 Protein-protein interactions for F9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZNF644  
Affinity Capture-MS Homo sapiens
2 EOGT 285203
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 TOR3A 64222
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 MCM8 84515
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 DDX11L8  
Affinity Capture-MS Homo sapiens
6 ZNHIT6 54680
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 ACOT9 23597
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 DSCC1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 HOXD13  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 FERMT2 10979
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 SETD2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 DPYSL4 10570
Affinity Capture-MS Homo sapiens
13 TUBB3 10381
Affinity Capture-MS Homo sapiens
14 CBWD1 55871
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 SMG8 55181
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 YY1 7528
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 APBB1  
Affinity Capture-MS Homo sapiens
18 TUBA1A 7846
Affinity Capture-MS Homo sapiens
19 MSTO1 55154
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 LRP1 4035
Reconstituted Complex Homo sapiens
21 ZNF195  
Affinity Capture-MS Homo sapiens
22 HDAC3 8841
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 UBR5 51366
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 ERF  
Affinity Capture-MS Homo sapiens
25 LRP5 4041
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 RBM14-RBM4 100526737
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 SENP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 TRMT44  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 LMLN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 MIB2 142678
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 KIAA1671  
Affinity Capture-MS Homo sapiens
32 OAF 220323
Affinity Capture-MS Homo sapiens
33 POGLUT1 56983
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 TMEM55A 55529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 DCP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 HELLS 3070
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 FIGNL1 63979
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which F9 is involved
PathwayEvidenceSource
Defective cofactor function of FVIIIa variant TAS Reactome
Defective F9 activation TAS Reactome
Defective F9 secretion TAS Reactome
Defective F9 variant does not activate FX TAS Reactome
Defective factor IX causes hemophilia B TAS Reactome
Defective factor IX causes thrombophilia TAS Reactome
Defective factor VIII causes hemophilia A TAS Reactome
Defective gamma-carboxylation of F9 TAS Reactome
Defects of contact activation system (CAS) and kallikrein/kinin system (KKS) TAS Reactome
Disease TAS Reactome
Diseases of hemostasis TAS Reactome
Extrinsic Pathway of Fibrin Clot Formation TAS Reactome
Formation of Fibrin Clot (Clotting Cascade) TAS Reactome
Gamma carboxylation, hypusinylation, hydroxylation, and arylsulfatase activation IEA Reactome
Gamma carboxylation, hypusinylation, hydroxylation, and arylsulfatase activation TAS Reactome
Gamma-carboxylation of protein precursors TAS Reactome
Gamma-carboxylation, transport, and amino-terminal cleavage of proteins TAS Reactome
Hemostasis TAS Reactome
Intrinsic Pathway of Fibrin Clot Formation TAS Reactome
Metabolism of proteins IEA Reactome
Metabolism of proteins TAS Reactome
Post-translational protein modification IEA Reactome
Post-translational protein modification TAS Reactome
Protein hydroxylation IEA Reactome
Removal of aminoterminal propeptides from gamma-carboxylated proteins TAS Reactome
Transport of gamma-carboxylated protein precursors from the endoplasmic reticulum to the Golgi apparatus TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here