Gene description for Thy1
Gene name thymus cell antigen 1, theta
Gene symbol Thy1
Other names/aliases CD90
T25
Thy-1
Thy-1.2
Thy1.1
Thy1.2
Species Mus musculus
 Database cross references - Thy1
ExoCarta ExoCarta_21838
Vesiclepedia VP_21838
Entrez Gene 21838
UniProt P01831  
 Thy1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Thy1
Molecular Function
    GTPase activator activity GO:0005096 IBA
    GTPase activator activity GO:0005096 ISO
    GTPase activator activity GO:0005096 ISS
    integrin binding GO:0005178 IMP
    integrin binding GO:0005178 ISO
    enzyme binding GO:0019899 ISO
    protein kinase binding GO:0019901 ISO
    GPI anchor binding GO:0034235 ISO
    GPI anchor binding GO:0034235 ISS
Biological Process
    angiogenesis GO:0001525 IDA
    regulation of cell-matrix adhesion GO:0001952 ISO
    positive regulation of cellular extravasation GO:0002693 IEA
    positive regulation of cellular extravasation GO:0002693 ISO
    negative regulation of protein kinase activity GO:0006469 ISS
    cytoskeleton organization GO:0007010 ISO
    cytoskeleton organization GO:0007010 ISS
    integrin-mediated signaling pathway GO:0007229 IBA
    integrin-mediated signaling pathway GO:0007229 IMP
    integrin-mediated signaling pathway GO:0007229 ISO
    cell-cell signaling GO:0007267 IDA
    cell-cell signaling GO:0007267 ISO
    negative regulation of cell migration GO:0030336 ISO
    negative regulation of cell migration GO:0030336 ISS
    heterotypic cell-cell adhesion GO:0034113 IEA
    heterotypic cell-cell adhesion GO:0034113 ISO
    receptor clustering GO:0043113 ISO
    receptor clustering GO:0043113 ISS
    positive regulation of GTPase activity GO:0043547 ISS
    retinal cone cell development GO:0046549 IMP
    focal adhesion assembly GO:0048041 ISO
    focal adhesion assembly GO:0048041 ISS
    negative regulation of axonogenesis GO:0050771 ISO
    negative regulation of axonogenesis GO:0050771 ISS
    T cell receptor signaling pathway GO:0050852 ISO
    T cell receptor signaling pathway GO:0050852 ISS
    negative regulation of T cell receptor signaling pathway GO:0050860 IDA
    positive regulation of T cell activation GO:0050870 ISO
    positive regulation of T cell activation GO:0050870 ISS
    positive regulation of release of sequestered calcium ion into cytosol GO:0051281 ISO
    positive regulation of release of sequestered calcium ion into cytosol GO:0051281 ISS
    positive regulation of focal adhesion assembly GO:0051894 IBA
    positive regulation of focal adhesion assembly GO:0051894 IMP
    positive regulation of focal adhesion assembly GO:0051894 ISO
    negative regulation of neuron projection regeneration GO:0070571 IMP
    negative regulation of neuron projection regeneration GO:0070571 ISO
    cell-cell adhesion GO:0098609 ISO
    cell-cell adhesion GO:0098609 ISS
    regulation of Rho-dependent protein serine/threonine kinase activity GO:2000298 IDA
Subcellular Localization
    endoplasmic reticulum GO:0005783 IEA
    endoplasmic reticulum GO:0005783 ISO
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    external side of plasma membrane GO:0009897 IBA
    external side of plasma membrane GO:0009897 IDA
    cell surface GO:0009986 ISO
    cell surface GO:0009986 ISS
    apical plasma membrane GO:0016324 IEA
    apical plasma membrane GO:0016324 ISO
    dendrite GO:0030425 IBA
    dendrite GO:0030425 IDA
    growth cone GO:0030426 ISO
    growth cone GO:0030426 ISS
    axolemma GO:0030673 ISO
    axolemma GO:0030673 ISS
    dendrite membrane GO:0032590 ISO
    dendrite membrane GO:0032590 ISS
    neuronal cell body membrane GO:0032809 ISO
    neuronal cell body membrane GO:0032809 ISS
    myelin sheath GO:0043209 HDA
    membrane raft GO:0045121 IBA
    membrane raft GO:0045121 IDA
    membrane raft GO:0045121 ISO
    presynapse GO:0098793 IDA
    presynapse GO:0098793 IMP
    postsynapse GO:0098794 IDA
    postsynapse GO:0098794 IMP
    glutamatergic synapse GO:0098978 IDA
    glutamatergic synapse GO:0098978 IMP
 Experiment description of studies that identified Thy1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Thy1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SNCA 6622
Affinity Capture-MS Homo sapiens
2 App 11820
Affinity Capture-MS Mus musculus
3 Kctd13  
Affinity Capture-MS Mus musculus
4 Dlg4  
Co-fractionation Mus musculus
5 Tia1  
Affinity Capture-MS Mus musculus
6 Fancd2  
Affinity Capture-MS Mus musculus
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