Gene description for HMHA1
Gene name histocompatibility (minor) HA-1
Gene symbol HMHA1
Other names/aliases ARHGAP45
HA-1
HLA-HA1
Species Homo sapiens
 Database cross references - HMHA1
ExoCarta ExoCarta_23526
Vesiclepedia VP_23526
Entrez Gene 23526
HGNC 17102
MIM 601155
UniProt Q92619  
 HMHA1 identified in sEVs derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Thymus 23844026    
 Gene ontology annotations for HMHA1
Molecular Function
    GTPase activator activity GO:0005096 IBA
    GTPase activator activity GO:0005096 TAS
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    signal transduction GO:0007165 IEA
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
    negative regulation of small GTPase mediated signal transduction GO:0051058 IBA
Subcellular Localization
    extracellular region GO:0005576 TAS
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    membrane GO:0016020 HDA
    membrane GO:0016020 IBA
    ruffle membrane GO:0032587 IEA
    secretory granule lumen GO:0034774 TAS
    azurophil granule lumen GO:0035578 TAS
 Experiment description of studies that identified HMHA1 in sEVs
1
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 217
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for HMHA1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATG16L1 55054
Affinity Capture-MS Homo sapiens
2 TRIM50  
Two-hybrid Homo sapiens
3 YWHAE 7531
Affinity Capture-MS Homo sapiens
4 GOLGA2 2801
Two-hybrid Homo sapiens
5 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
6 AURKA 6790
Affinity Capture-MS Homo sapiens
7 HSF2BP  
Two-hybrid Homo sapiens
8 RINT1 60561
Two-hybrid Homo sapiens
9 ZUFSP 221302
Two-hybrid Homo sapiens
10 HNRNPCL1 343069
Co-fractionation Homo sapiens
11 MDM2  
Affinity Capture-MS Homo sapiens
12 NR3C1 2908
Proximity Label-MS Homo sapiens
13 DDX58 23586
Affinity Capture-RNA Homo sapiens
14 BICD2 23299
Two-hybrid Homo sapiens
15 BRD4 23476
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here