Gene description for Pebp1
Gene name phosphatidylethanolamine binding protein 1
Gene symbol Pebp1
Other names/aliases HCNP
Pbp
Pbp1
Pbpr
Rkip
Species Mus musculus
 Database cross references - Pebp1
ExoCarta ExoCarta_23980
Vesiclepedia VP_23980
Entrez Gene 23980
UniProt P70296  
 Pebp1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mov neuroglial cells 15210972    
Oligodendrocytes 21136642    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Pebp1
Molecular Function
    serine-type endopeptidase inhibitor activity GO:0004867 IEA
    signaling receptor binding GO:0005102 ISO
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    lipid binding GO:0008289 IEA
    enzyme binding GO:0019899 ISO
    kinase binding GO:0019900 ISO
    protein kinase binding GO:0019901 ISO
    receptor serine/threonine kinase binding GO:0033612 ISO
    mitogen-activated protein kinase binding GO:0051019 ISO
Biological Process
    MAPK cascade GO:0000165 ISO
    negative regulation of protein phosphorylation GO:0001933 ISO
    regulation of the force of heart contraction GO:0002026 ISO
    negative regulation of MAPK cascade GO:0043409 IBA
    negative regulation of MAPK cascade GO:0043409 ISO
    positive regulation of cAMP-mediated signaling GO:0043950 ISO
    positive regulation of mitotic nuclear division GO:0045840 ISO
    sperm capacitation GO:0048240 IDA
    positive regulation of acetylcholine metabolic process GO:0060409 ISO
    positive regulation of acetylcholine biosynthetic process GO:1905923 ISO
Subcellular Localization
    extracellular space GO:0005615 ISO
    cytoplasm GO:0005737 IEA
    mitochondrial outer membrane GO:0005741 ISO
    synaptic vesicle GO:0008021 ISO
    cell surface GO:0009986 IDA
    cell surface GO:0009986 ISO
    neuronal cell body GO:0043025 ISO
    myelin sheath GO:0043209 HDA
    axon terminus GO:0043679 ISO
    apical part of cell GO:0045177 ISO
 Experiment description of studies that identified Pebp1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 39
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|HSC70|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15210972    
Organism Mus musculus
Experiment description Cells release prions in association with exosomes.
Authors "Fevrier B, Vilette D, Archer F, Loew D, Faigle W, Vidal M, Laude H, Raposo G"
Journal name PNAS
Publication year 2004
Sample Mov neuroglial cells
Sample name Mov neuroglial cell
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Western blotting
Immunoelectron Microscopy
3
Experiment ID 33
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21136642    
Organism Mus musculus
Experiment description Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons?
Authors "Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J"
Journal name PROTEOMICS_CL
Publication year 2007
Sample Oligodendrocytes
Sample name Oligodendrocytes
Oli-neu
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.14 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
4
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Pebp1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Invs  
Affinity Capture-MS Mus musculus
2 Map2k1 26395
Affinity Capture-Western Mus musculus
3 Tmem173  
Proximity Label-MS Mus musculus
4 Kctd13  
Affinity Capture-MS Mus musculus
5 Tfe3  
Affinity Capture-MS Mus musculus
6 Fancd2  
Affinity Capture-MS Mus musculus
7 PPARD  
Reconstituted Complex Homo sapiens
8 Raf1  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
9 Kcnma1  
Affinity Capture-MS Mus musculus
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