Gene description for Rfwd2
Gene name ring finger and WD repeat domain 2
Gene symbol Rfwd2
Other names/aliases AI316802
C80879
Cop1
Species Mus musculus
 Database cross references - Rfwd2
ExoCarta ExoCarta_26374
Vesiclepedia VP_26374
Entrez Gene 26374
UniProt Q9R1A8  
 Rfwd2 identified in sEVs derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Rfwd2
Molecular Function
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    ubiquitin protein ligase activity GO:0061630 IBA
    ubiquitin protein ligase activity GO:0061630 IEA
    ubiquitin protein ligase activity GO:0061630 ISO
Biological Process
    response to ionizing radiation GO:0010212 IEA
    response to ionizing radiation GO:0010212 ISO
    protein ubiquitination GO:0016567 IEA
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 IEA
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 ISO
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IEA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 ISO
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    Golgi membrane GO:0000139 ISO
    nuclear speck GO:0016607 IEA
    Cul4A-RING E3 ubiquitin ligase complex GO:0031464 IEA
    Cul4A-RING E3 ubiquitin ligase complex GO:0031464 ISO
 Experiment description of studies that identified Rfwd2 in sEVs
1
Experiment ID 15
MISEV standards
EM
Biophysical techniques
CD63
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Rfwd2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Trib2  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
2 Trib1  
Affinity Capture-Western Mus musculus
3 Reg1  
Affinity Capture-Western Mus musculus
4 Fasn 14104
Affinity Capture-Western Mus musculus
5 Trib3  
Affinity Capture-Western Mus musculus
6 Fitm2  
Positive Genetic Mus musculus
7 UBC 7316
Affinity Capture-Western Homo sapiens
8 Cul4b  
Affinity Capture-Western Mus musculus
9 Etv5  
Affinity Capture-Western Mus musculus
10 Ptpn11 19247
Affinity Capture-Western Mus musculus
11 Cebpd  
Affinity Capture-Western Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here