Gene description for Iqgap1
Gene name IQ motif containing GTPase activating protein 1
Gene symbol Iqgap1
Other names/aliases AA682088
D7Ertd237e
D7Ertd257e
mKIAA0051
Species Mus musculus
 Database cross references - Iqgap1
ExoCarta ExoCarta_29875
Vesiclepedia VP_29875
Entrez Gene 29875
UniProt Q9JKF1  
 Iqgap1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
Mast cells 17486113    
Microglia 16081791    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Iqgap1
Molecular Function
    MAP-kinase scaffold activity GO:0005078 IEA
    MAP-kinase scaffold activity GO:0005078 ISO
    GTPase activator activity GO:0005096 IBA
    calcium ion binding GO:0005509 IEA
    calcium ion binding GO:0005509 ISO
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IBA
    calmodulin binding GO:0005516 IEA
    calmodulin binding GO:0005516 ISO
    phosphatidylinositol-3,4,5-trisphosphate binding GO:0005547 ISO
    phosphatidylinositol-3,4,5-trisphosphate binding GO:0005547 ISS
    protein kinase binding GO:0019901 ISO
    protein phosphatase binding GO:0019903 IEA
    protein phosphatase binding GO:0019903 ISO
    protein domain specific binding GO:0019904 IEA
    protein domain specific binding GO:0019904 ISO
    small GTPase binding GO:0031267 IPI
    small GTPase binding GO:0031267 ISO
    protein serine/threonine kinase activator activity GO:0043539 IEA
    protein serine/threonine kinase activator activity GO:0043539 ISO
    S100 protein binding GO:0044548 IEA
    S100 protein binding GO:0044548 ISO
    protein-containing complex binding GO:0044877 IEA
    protein-containing complex binding GO:0044877 ISO
    actin filament binding GO:0051015 IBA
    mitogen-activated protein kinase binding GO:0051019 IEA
    mitogen-activated protein kinase binding GO:0051019 ISO
    molecular adaptor activity GO:0060090 ISO
Biological Process
    MAPK cascade GO:0000165 IEA
    regulation of cytokine production GO:0001817 IMP
    epidermal growth factor receptor signaling pathway GO:0007173 IBA
    epidermal growth factor receptor signaling pathway GO:0007173 IMP
    epidermal growth factor receptor signaling pathway GO:0007173 ISO
    regulation of mitotic cell cycle GO:0007346 ISO
    regulation of mitotic cell cycle GO:0007346 ISS
    fibroblast growth factor receptor signaling pathway GO:0008543 IMP
    fibroblast migration GO:0010761 IBA
    fibroblast migration GO:0010761 IGI
    cell migration GO:0016477 ISO
    negative regulation of dephosphorylation GO:0035305 IGI
    cellular response to platelet-derived growth factor stimulus GO:0036120 IMP
    positive regulation of MAPK cascade GO:0043410 IEA
    positive regulation of MAPK cascade GO:0043410 ISO
    cellular response to fibroblast growth factor stimulus GO:0044344 IMP
    positive regulation of protein kinase activity GO:0045860 IMP
    positive regulation of protein kinase activity GO:0045860 ISO
    platelet-derived growth factor receptor signaling pathway GO:0048008 IMP
    positive regulation of focal adhesion assembly GO:0051894 IEA
    positive regulation of focal adhesion assembly GO:0051894 ISO
    caveola assembly GO:0070836 IMP
    cellular response to calcium ion GO:0071277 IEA
    cellular response to calcium ion GO:0071277 ISO
    cellular response to epidermal growth factor stimulus GO:0071364 IMP
    cellular response to epidermal growth factor stimulus GO:0071364 ISO
    podocyte development GO:0072015 ISS
    regulation of postsynapse assembly GO:0150052 IEA
    positive regulation of dendrite development GO:1900006 IEA
    positive regulation of dendrite development GO:1900006 ISO
    positive regulation of peptidyl-tyrosine autophosphorylation GO:1900086 ISO
    mitotic actomyosin contractile ring assembly actin filament organization GO:1903479 IBA
    positive regulation of protein localization GO:1903829 IEA
    positive regulation of protein localization GO:1903829 ISO
    positive regulation of vascular associated smooth muscle cell migration GO:1904754 IEA
    positive regulation of vascular associated smooth muscle cell migration GO:1904754 ISO
    neuron projection extension GO:1990138 IEA
    neuron projection extension GO:1990138 ISO
    response to angiotensin GO:1990776 IEA
Subcellular Localization
    ruffle GO:0001726 IEA
    ruffle GO:0001726 ISO
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    microtubule GO:0005874 IEA
    microtubule GO:0005874 ISO
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    cell-cell junction GO:0005911 IDA
    focal adhesion GO:0005925 IDA
    focal adhesion GO:0005925 ISO
    cell cortex GO:0005938 IBA
    cytoplasmic side of plasma membrane GO:0009898 ISO
    cytoplasmic side of plasma membrane GO:0009898 ISS
    actin cytoskeleton GO:0015629 ISO
    actin cytoskeleton GO:0015629 ISS
    microtubule cytoskeleton GO:0015630 ISO
    microtubule cytoskeleton GO:0015630 ISS
    basolateral plasma membrane GO:0016323 IDA
    apical plasma membrane GO:0016324 IDA
    apical plasma membrane GO:0016324 ISO
    lateral plasma membrane GO:0016328 IDA
    axon GO:0030424 ISO
    axon GO:0030424 ISS
    growth cone GO:0030426 ISO
    growth cone GO:0030426 ISS
    midbody GO:0030496 IEA
    midbody GO:0030496 ISO
    cortical actin cytoskeleton GO:0030864 IPI
    cell leading edge GO:0031252 IDA
    cell leading edge GO:0031252 IGI
    protein-containing complex GO:0032991 ISO
    slit diaphragm GO:0036057 ISS
    cytoplasmic ribonucleoprotein granule GO:0036464 IEA
    cytoplasmic ribonucleoprotein granule GO:0036464 ISO
    neuron projection GO:0043005 IGI
    neuron projection GO:0043005 ISO
    neuron projection GO:0043005 ISS
    postsynapse GO:0098794 IEA
    ribonucleoprotein complex GO:1990904 IDA
    ribonucleoprotein complex GO:1990904 IGI
 Experiment description of studies that identified Iqgap1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
6
Experiment ID 14
MISEV standards
✔
EM
Biophysical techniques
✔
RAB7|RAB11|CD9|CD63|LAMP1|LAMP2
Enriched markers
✔
DNM
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16081791    
Organism Mus musculus
Experiment description Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism.
Authors "Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L"
Journal name JIMMU
Publication year 2005
Sample Microglia
Sample name N9
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
7
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Iqgap1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Elf5  
Affinity Capture-MS Mus musculus
2 Mycbp2 105689
Affinity Capture-MS Mus musculus
3 Eef1e1  
Co-fractionation Mus musculus
4 Klhl40  
Affinity Capture-MS Mus musculus
5 Syngap1  
Affinity Capture-MS Mus musculus
6 Atxn1  
Affinity Capture-MS Mus musculus
7 Foxp3  
Affinity Capture-MS Mus musculus
8 Kctd13  
Affinity Capture-MS Mus musculus
9 Ncl 17975
Co-fractionation Mus musculus
10 Mettl21e  
Affinity Capture-MS Mus musculus
11 Rock2  
Co-fractionation Mus musculus
12 Lsm14a  
Co-fractionation Mus musculus
13 Agap2  
Affinity Capture-MS Mus musculus
14 Eif4g1 208643
Co-fractionation Mus musculus
15 Dync1i1  
Co-fractionation Mus musculus
16 Ina  
Co-fractionation Mus musculus
17 Dmrt2  
Affinity Capture-MS Mus musculus
18 Syncrip 56403
Co-fractionation Mus musculus
19 Ctsh  
Co-fractionation Mus musculus
20 Eed  
Affinity Capture-MS Mus musculus
21 Mapt  
Co-fractionation Mus musculus
22 Stau1  
Co-fractionation Mus musculus
23 Fancd2  
Affinity Capture-MS Mus musculus
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