Gene description for ARHGAP5
Gene name Rho GTPase activating protein 5
Gene symbol ARHGAP5
Other names/aliases GFI2
RhoGAP5
p190-B
p190BRhoGAP
Species Homo sapiens
 Database cross references - ARHGAP5
ExoCarta ExoCarta_394
Vesiclepedia VP_394
Entrez Gene 394
HGNC 675
MIM 602680
UniProt Q13017  
 ARHGAP5 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for ARHGAP5
Molecular Function
    GTPase activity GO:0003924 TAS
    GTPase activator activity GO:0005096 IBA
    GTPase activator activity GO:0005096 TAS
    protein binding GO:0005515 IPI
    SH2 domain binding GO:0042169 IPI
Biological Process
    positive regulation of mesenchymal cell proliferation GO:0002053 IEA
    cell adhesion GO:0007155 TAS
    Rho protein signal transduction GO:0007266 IBA
    regulation of cell size GO:0008361 IBA
    epithelial cell migration GO:0010631 IEA
    positive regulation of epithelial cell migration GO:0010634 IEA
    mammary gland development GO:0030879 IEA
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
Subcellular Localization
    cytoplasm GO:0005737 TAS
    endoplasmic reticulum GO:0005783 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IEA
 Experiment description of studies that identified ARHGAP5 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ARHGAP5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PTPRU  
Proximity Label-MS Homo sapiens
2 FAM134C 162427
Affinity Capture-MS Homo sapiens
3 PARP1 142
Proximity Label-MS Homo sapiens
4 TP53 7157
Affinity Capture-MS Homo sapiens
5 RND1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
6 UNK  
Affinity Capture-RNA Homo sapiens
7 PFN1 5216
Proximity Label-MS Homo sapiens
8 ZYX 7791
Affinity Capture-MS Homo sapiens
9 CTNNB1 1499
Proximity Label-MS Homo sapiens
10 ADRB2  
PCA Homo sapiens
Affinity Capture-Luminescence Homo sapiens
11 SMURF1 57154
Affinity Capture-MS Homo sapiens
12 IQCF1  
Affinity Capture-MS Homo sapiens
13 KAZN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 MGA  
Affinity Capture-MS Homo sapiens
15 RND3 390
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
16 CDH1 999
Proximity Label-MS Homo sapiens
17 RPA3 6119
Proximity Label-MS Homo sapiens
18 PXN 5829
Affinity Capture-Western Homo sapiens
19 DYNLRB1 83658
Proximity Label-MS Homo sapiens
20 PLK1 5347
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 RND2  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
22 EPHA2 1969
Proximity Label-MS Homo sapiens
23 XRCC6 2547
Co-fractionation Homo sapiens
24 RHOB 388
Proximity Label-MS Homo sapiens
25 SMURF2 64750
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 DOCK11 139818
Affinity Capture-MS Homo sapiens
27 RHOA 387
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
FRET Homo sapiens
28 DOCK3  
Affinity Capture-MS Homo sapiens
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