Gene description for LRP6
Gene name low density lipoprotein receptor-related protein 6
Gene symbol LRP6
Other names/aliases ADCAD2
Species Homo sapiens
 Database cross references - LRP6
ExoCarta ExoCarta_4040
Vesiclepedia VP_4040
Entrez Gene 4040
HGNC 6698
MIM 603507
UniProt O75581  
 LRP6 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 34887515    
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for LRP6
Molecular Function
    low-density lipoprotein particle receptor activity GO:0005041 IDA
    signaling receptor binding GO:0005102 IPI
    frizzled binding GO:0005109 IPI
    protein binding GO:0005515 IPI
    coreceptor activity GO:0015026 IDA
    coreceptor activity GO:0015026 NAS
    Wnt-protein binding GO:0017147 IPI
    Wnt-protein binding GO:0017147 TAS
    kinase inhibitor activity GO:0019210 IMP
    toxin transmembrane transporter activity GO:0019534 IMP
    toxin transmembrane transporter activity GO:0019534 IMP
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IPI
Biological Process
    endocytosis GO:0006897 IEA
    positive regulation of cytosolic calcium ion concentration GO:0007204 IEA
    chemical synaptic transmission GO:0007268 IEA
    nervous system development GO:0007399 IBA
    neural crest formation GO:0014029 IDA
    neural crest cell differentiation GO:0014033 IDA
    Wnt signaling pathway GO:0016055 IDA
    negative regulation of smooth muscle cell apoptotic process GO:0034392 IMP
    response to peptide hormone GO:0043434 IEA
    positive regulation of cell cycle GO:0045787 IMP
    positive regulation of DNA-templated transcription GO:0045893 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of DNA-binding transcription factor activity GO:0051091 IDA
    transmembrane transport GO:0055085 IEA
    canonical Wnt signaling pathway GO:0060070 IDA
    canonical Wnt signaling pathway GO:0060070 IDA
    canonical Wnt signaling pathway GO:0060070 IGI
    canonical Wnt signaling pathway GO:0060070 IMP
    canonical Wnt signaling pathway GO:0060070 TAS
    cellular response to cholesterol GO:0071397 IMP
    dopaminergic neuron differentiation GO:0071542 ISS
    negative regulation of protein serine/threonine kinase activity GO:0071901 IDA
    protein localization to plasma membrane GO:0072659 IPI
    cell-cell adhesion GO:0098609 IEA
    midbrain dopaminergic neuron differentiation GO:1904948 TAS
Subcellular Localization
    extracellular region GO:0005576 TAS
    early endosome GO:0005769 IDA
    endoplasmic reticulum GO:0005783 IEA
    Golgi apparatus GO:0005794 IDA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    caveola GO:0005901 IDA
    cell surface GO:0009986 IDA
    cytoplasmic vesicle GO:0031410 IDA
    early endosome membrane GO:0031901 TAS
    neuronal cell body GO:0043025 IEA
    synapse GO:0045202 IEA
    Wnt-Frizzled-LRP5/6 complex GO:1990851 IDA
    Wnt-Frizzled-LRP5/6 complex GO:1990851 IPI
    Wnt-Frizzled-LRP5/6 complex GO:1990851 TAS
    Wnt signalosome GO:1990909 IDA
    Wnt signalosome GO:1990909 NAS
 Experiment description of studies that identified LRP6 in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 1203
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
9
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Antibody array
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for LRP6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KLK15  
Affinity Capture-MS Homo sapiens
2 ETV6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 KLK5 25818
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 GJA1 2697
Proximity Label-MS Homo sapiens
5 ATP13A2  
Two-hybrid Homo sapiens
6 CSNK1A1 1452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 CFC1  
Affinity Capture-MS Homo sapiens
8 ECEL1  
Affinity Capture-MS Homo sapiens
9 GAS8  
Co-fractionation Homo sapiens
10 TLR1 7096
Affinity Capture-MS Homo sapiens
11 KLK2  
Affinity Capture-MS Homo sapiens
12 GSK3A 2931
Two-hybrid Homo sapiens
13 B3GAT1  
Proximity Label-MS Homo sapiens
14 CTNNB1 1499
Affinity Capture-Western Homo sapiens
15 ZNRF3  
Affinity Capture-Western Homo sapiens
16 IQGAP1 8826
Affinity Capture-Western Homo sapiens
17 CLEC2B  
Affinity Capture-MS Homo sapiens
18 LGR4 55366
Affinity Capture-Western Homo sapiens
19 NCR3  
Affinity Capture-MS Homo sapiens
20 ST8SIA5  
Affinity Capture-MS Homo sapiens
21 SOST  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 SERPINF1 5176
Reconstituted Complex Homo sapiens
23 ST8SIA4 7903
Affinity Capture-MS Homo sapiens
24 BAGE2  
Affinity Capture-MS Homo sapiens
25 BTNL2  
Affinity Capture-MS Homo sapiens
26 SYT9 143425
Affinity Capture-MS Homo sapiens
27 GSK3B 2932
Biochemical Activity Homo sapiens
Far Western Homo sapiens
28 HLA-G 3135
Affinity Capture-MS Homo sapiens
29 TAZ  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 ZKSCAN8  
Affinity Capture-MS Homo sapiens
31 NPC1 4864
Affinity Capture-Western Homo sapiens
32 FZD8 8325
Reconstituted Complex Homo sapiens
33 PAPD7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 CRISP2  
Affinity Capture-MS Homo sapiens
35 ADAMTS12 81792
Affinity Capture-MS Homo sapiens
36 DHH  
Affinity Capture-MS Homo sapiens
37 B3GAT2  
Affinity Capture-MS Homo sapiens
38 LYPD1  
Affinity Capture-MS Homo sapiens
39 HGF 3082
Affinity Capture-MS Homo sapiens
40 TGIF2LY  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 FAM19A2 338811
Affinity Capture-MS Homo sapiens
42 GRIN2A  
Affinity Capture-MS Homo sapiens
43 SDF2L1 23753
Affinity Capture-MS Homo sapiens
44 ELOVL5 60481
Proximity Label-MS Homo sapiens
45 SEC61B 10952
Proximity Label-MS Homo sapiens
46 DKK2  
Affinity Capture-MS Homo sapiens
47 NMS  
Affinity Capture-MS Homo sapiens
48 ADAMTS4  
Affinity Capture-MS Homo sapiens
49 PRG3  
Affinity Capture-MS Homo sapiens
50 WNT3A 89780
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
51 CMA1  
Affinity Capture-MS Homo sapiens
52 GGH 8836
Affinity Capture-MS Homo sapiens
53 APOB 338
Reconstituted Complex Homo sapiens
54 CBLN4  
Affinity Capture-MS Homo sapiens
55 WDR25  
Affinity Capture-MS Homo sapiens
56 PTPRH 5794
Proximity Label-MS Homo sapiens
57 PRG2  
Affinity Capture-MS Homo sapiens
58 TRAF6 7189
Affinity Capture-Western Homo sapiens
59 PI15  
Affinity Capture-MS Homo sapiens
60 RASSF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 WNT5A 7474
Affinity Capture-Western Homo sapiens
62 LYZL1  
Affinity Capture-MS Homo sapiens
63 IL5RA  
Affinity Capture-MS Homo sapiens
64 CTSG 1511
Affinity Capture-MS Homo sapiens
65 KCNE3  
Affinity Capture-MS Homo sapiens
66 DNMT3B  
Affinity Capture-MS Homo sapiens
67 RASD2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 GPATCH2  
Affinity Capture-MS Homo sapiens
69 EPN1 29924
Affinity Capture-Western Homo sapiens
70 FZD5 7855
Affinity Capture-Western Homo sapiens
71 GXYLT1 283464
Affinity Capture-MS Homo sapiens
72 EDF1 8721
Affinity Capture-MS Homo sapiens
73 RHOB 388
Proximity Label-MS Homo sapiens
74 EDN3  
Affinity Capture-MS Homo sapiens
75 PARP16 54956
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 DKK1 22943
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
77 ADAM30 11085
Affinity Capture-MS Homo sapiens
78 WNT1  
Reconstituted Complex Homo sapiens
79 CSNK1A1L 122011
Affinity Capture-MS Homo sapiens
80 SCGB1D1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 FCGRT 2217
Affinity Capture-MS Homo sapiens
82 ADAM33 80332
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 MGAT4C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 WNT10B 7480
Affinity Capture-MS Homo sapiens
85 OVOL2  
Affinity Capture-MS Homo sapiens
86 AXIN1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
87 ZNF383  
Affinity Capture-MS Homo sapiens
88 ST3GAL4 6484
Affinity Capture-MS Homo sapiens
89 SIRPD  
Affinity Capture-MS Homo sapiens
90 LRRK2 120892
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
91 PRSS37  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 POMK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 WISP3  
Affinity Capture-MS Homo sapiens
94 P2RX2  
Affinity Capture-MS Homo sapiens
95 CHST9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 PODN 127435
Affinity Capture-MS Homo sapiens
97 CAV1 857
Proximity Label-MS Homo sapiens
98 DKKL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 KLK3  
Affinity Capture-MS Homo sapiens
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