Gene description for ARHGEF3
Gene name Rho guanine nucleotide exchange factor (GEF) 3
Gene symbol ARHGEF3
Other names/aliases GEF3
STA3
XPLN
Species Homo sapiens
 Database cross references - ARHGEF3
ExoCarta ExoCarta_50650
Vesiclepedia VP_50650
Entrez Gene 50650
HGNC 683
MIM 612115
UniProt Q9NR81  
 ARHGEF3 identified in sEVs derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for ARHGEF3
Molecular Function
    guanyl-nucleotide exchange factor activity GO:0005085 TAS
    protein binding GO:0005515 IPI
Biological Process
    Rho protein signal transduction GO:0007266 TAS
    positive regulation of Rho protein signal transduction GO:0035025 IBA
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
 Experiment description of studies that identified ARHGEF3 in sEVs
1
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for ARHGEF3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NXF1 10482
Affinity Capture-RNA Homo sapiens
2 APEX1 328
Affinity Capture-RNA Homo sapiens
3 RHOB 388
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
4 PBX4  
Two-hybrid Homo sapiens
5 TERF1 7013
Two-hybrid Homo sapiens
6 HSF2BP  
Two-hybrid Homo sapiens
7 RHOA 387
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
8 CEP70  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
9 TRIM23 373
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
10 SHMT2 6472
Affinity Capture-RNA Homo sapiens
11 XPO1 7514
Affinity Capture-MS Homo sapiens
12 TRIM27  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here