Gene description for Nt5c
Gene name 5',3'-nucleotidase, cytosolic
Gene symbol Nt5c
Other names/aliases Dnt
Dnt1
Umph-2
Umph2
Species Mus musculus
 Database cross references - Nt5c
ExoCarta ExoCarta_50773
Vesiclepedia VP_50773
Entrez Gene 50773
UniProt Q9JM14  
 Nt5c identified in sEVs derived from the following tissue/cell type
Melanoma cells 34957415    
Melanoma cells 34957415    
 Gene ontology annotations for Nt5c
Molecular Function
    nucleotidase activity GO:0008252 ISO
    5'-nucleotidase activity GO:0008253 IBA
    5'-nucleotidase activity GO:0008253 IDA
    phosphatase activity GO:0016791 IDA
    pyrimidine nucleotide binding GO:0019103 IEA
    pyrimidine nucleotide binding GO:0019103 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    metal ion binding GO:0046872 IEA
    IMP 5'-nucleotidase activity GO:0050483 IDA
Biological Process
    allantoin metabolic process GO:0000255 IDA
    IMP catabolic process GO:0006204 IDA
    dCMP catabolic process GO:0006249 IDA
    pyrimidine deoxyribonucleotide catabolic process GO:0009223 IBA
    pyrimidine deoxyribonucleotide catabolic process GO:0009223 ISO
    deoxyribonucleotide catabolic process GO:0009264 IDA
    dephosphorylation GO:0016311 ISO
    amide catabolic process GO:0043605 IDA
    UMP catabolic process GO:0046050 IDA
    dGMP catabolic process GO:0046055 IDA
    dTMP catabolic process GO:0046074 IDA
    dUMP catabolic process GO:0046079 IDA
Subcellular Localization
    nucleus GO:0005634 IEA
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 HDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISO
 Experiment description of studies that identified Nt5c in sEVs
1
Experiment ID 1117
MISEV standards
Biophysical techniques
Cd81|Icam1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 1118
MISEV standards
Biophysical techniques
Cd81|Icam1
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34957415    
Organism Mus musculus
Experiment description Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
Authors "García-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H."
Journal name Nat Cancer
Publication year 2021
Sample Melanoma cells
Sample name B16-F1R2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Nt5c
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Bax 12028
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Nt5c is involved
PathwayEvidenceSource
Metabolism IEA Reactome
Metabolism of nucleotides IEA Reactome
Nucleotide catabolism IEA Reactome
Purine catabolism IEA Reactome
Pyrimidine catabolism IEA Reactome





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