Gene description for NELFCD
Gene name negative elongation factor complex member C/D
Gene symbol NELFCD
Other names/aliases HSPC130
NELF-C
NELF-D
TH1
TH1L
Species Homo sapiens
 Database cross references - NELFCD
ExoCarta ExoCarta_51497
Vesiclepedia VP_51497
Entrez Gene 51497
HGNC 15934
MIM 605297
UniProt Q8IXH7  
 NELFCD identified in sEVs derived from the following tissue/cell type
Thymus 23844026    
 Gene ontology annotations for NELFCD
Molecular Function
    RNA binding GO:0003723 IBA
    protein binding GO:0005515 IPI
Biological Process
    negative regulation of transcription elongation by RNA polymerase II GO:0034244 IBA
    negative regulation of transcription elongation by RNA polymerase II GO:0034244 IDA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    membrane GO:0016020 HDA
    NELF complex GO:0032021 IBA
    NELF complex GO:0032021 IDA
    NELF complex GO:0032021 IPI
 Experiment description of studies that identified NELFCD in sEVs
1
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NELFCD
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RIT1 6016
Negative Genetic Homo sapiens
2 MCM7 4176
Affinity Capture-MS Homo sapiens
3 NCBP1 4686
Affinity Capture-MS Homo sapiens
4 TMBIM6 7009
Affinity Capture-MS Homo sapiens
5 BRDT  
Affinity Capture-MS Homo sapiens
6 ARPC4 10093
Two-hybrid Homo sapiens
7 PRSS3 5646
Affinity Capture-MS Homo sapiens
8 UBE3A 7337
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
9 C7orf76  
Affinity Capture-MS Homo sapiens
10 N4BP2L2 10443
Affinity Capture-MS Homo sapiens
11 COX8A  
Proximity Label-MS Homo sapiens
12 APEX1 328
Proximity Label-MS Homo sapiens
13 IGHMBP2 3508
Affinity Capture-MS Homo sapiens
14 COPB2 9276
Affinity Capture-MS Homo sapiens
15 Nelfa  
Affinity Capture-MS Mus musculus
16 NETO2  
Affinity Capture-MS Homo sapiens
17 XYLT1 64131
Affinity Capture-MS Homo sapiens
18 BRD4 23476
Affinity Capture-MS Homo sapiens
19 POLR2C 5432
Affinity Capture-MS Homo sapiens
20 SERF2 10169
Two-hybrid Homo sapiens
21 RPS11 6205
Affinity Capture-MS Homo sapiens
22 LAMTOR1 55004
Proximity Label-MS Homo sapiens
23 POLR2K  
Affinity Capture-MS Homo sapiens
24 ATP1A1 476
Affinity Capture-MS Homo sapiens
25 CCAR2 57805
Affinity Capture-MS Homo sapiens
26 SUPT5H 6829
Affinity Capture-MS Homo sapiens
27 SRSF6 6431
Affinity Capture-MS Homo sapiens
28 ZNF638 27332
Two-hybrid Homo sapiens
29 TUBA1B 10376
Affinity Capture-MS Homo sapiens
30 NELFE 7936
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 NTRK1 4914
Affinity Capture-MS Homo sapiens
32 DNAJA1 3301
Two-hybrid Homo sapiens
33 PEX14 5195
Proximity Label-MS Homo sapiens
34 HIST1H1C 3006
Affinity Capture-MS Homo sapiens
35 SRSF1 6426
Affinity Capture-MS Homo sapiens
36 DHFRL1  
Proximity Label-MS Homo sapiens
37 OGT 8473
Reconstituted Complex Homo sapiens
38 COX4I1 1327
Affinity Capture-MS Homo sapiens
39 POLR2B 5431
Affinity Capture-MS Homo sapiens
40 Pafah1b1 18472
Affinity Capture-MS Mus musculus
41 COX5B 1329
Affinity Capture-MS Homo sapiens
42 CACNA1A  
Two-hybrid Homo sapiens
43 EIF4G1 1981
Affinity Capture-MS Homo sapiens
44 POLR2E 5434
Affinity Capture-MS Homo sapiens
45 HYPK 25764
Affinity Capture-MS Homo sapiens
46 YWHAZ 7534
Affinity Capture-MS Homo sapiens
47 HDAC11  
Two-hybrid Homo sapiens
48 EPRS 2058
Two-hybrid Homo sapiens
49 PYCR2 29920
Cross-Linking-MS (XL-MS) Homo sapiens
50 PPP2CA 5515
Affinity Capture-MS Homo sapiens
51 PRMT5 10419
Two-hybrid Homo sapiens
52 TPM1 7168
Co-fractionation Homo sapiens
53 FABP5 2171
Affinity Capture-MS Homo sapiens
54 COX6C 1345
Affinity Capture-MS Homo sapiens
55 FOXL1  
Affinity Capture-MS Homo sapiens
56 RPS14 6208
Affinity Capture-MS Homo sapiens
57 DNPEP 23549
Affinity Capture-MS Homo sapiens
58 FECH 2235
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 HAX1  
Proximity Label-MS Homo sapiens
60 COX2 4513
Affinity Capture-MS Homo sapiens
61 NELFB 25920
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 ARAF 369
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
63 PSMB1 5689
Co-fractionation Homo sapiens
64 ACBD5 91452
Proximity Label-MS Homo sapiens
65 PHB2 11331
Affinity Capture-MS Homo sapiens
66 MUL1  
Affinity Capture-MS Homo sapiens
67 POLR2F  
Affinity Capture-MS Homo sapiens
68 HMGB1 3146
Co-fractionation Homo sapiens
69 TP53 7157
Synthetic Growth Defect Homo sapiens
70 MTA1 9112
Two-hybrid Homo sapiens
71 GSTM4 2948
Two-hybrid Homo sapiens
72 COX5A 9377
Affinity Capture-MS Homo sapiens
73 CDSN 1041
Two-hybrid Homo sapiens
74 SQSTM1 8878
Proximity Label-MS Homo sapiens
75 SMAD4  
Two-hybrid Homo sapiens
76 NELFA  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
77 ASNS 440
Affinity Capture-MS Homo sapiens
78 RPS26 6231
Affinity Capture-MS Homo sapiens
79 ANAPC2 29882
Proximity Label-MS Homo sapiens
80 NUP50 10762
Proximity Label-MS Homo sapiens
81 Lck 16818
Affinity Capture-MS Mus musculus
82 COX6B1 1340
Affinity Capture-MS Homo sapiens
83 SERBP1 26135
Affinity Capture-MS Homo sapiens
84 KIR2DL4  
Affinity Capture-MS Homo sapiens
85 KRAS 3845
Negative Genetic Homo sapiens
86 AR 367
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
87 VIM 7431
Affinity Capture-MS Homo sapiens
88 SPTLC2 9517
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which NELFCD is involved
PathwayEvidenceSource
Abortive elongation of HIV-1 transcript in the absence of Tat IEA Reactome
Disease IEA Reactome
Disease TAS Reactome
Formation of HIV elongation complex in the absence of HIV Tat IEA Reactome
Formation of HIV-1 elongation complex containing HIV-1 Tat TAS Reactome
Formation of HIV-1 elongation complex containing HIV-1 Tat IEA Reactome
Formation of RNA Pol II elongation complex TAS Reactome
Formation of the Early Elongation Complex TAS Reactome
Formation of the HIV-1 Early Elongation Complex IEA Reactome
Gene expression (Transcription) TAS Reactome
Generic Transcription Pathway TAS Reactome
HIV elongation arrest and recovery IEA Reactome
HIV Infection IEA Reactome
HIV Infection TAS Reactome
HIV Life Cycle IEA Reactome
HIV Life Cycle TAS Reactome
HIV Transcription Elongation IEA Reactome
HIV Transcription Elongation TAS Reactome
Infectious disease IEA Reactome
Infectious disease TAS Reactome
Late Phase of HIV Life Cycle IEA Reactome
Late Phase of HIV Life Cycle TAS Reactome
Pausing and recovery of HIV elongation IEA Reactome
Pausing and recovery of Tat-mediated HIV elongation IEA Reactome
RNA Polymerase II Pre-transcription Events TAS Reactome
RNA Polymerase II Transcription TAS Reactome
RNA Polymerase II Transcription Elongation TAS Reactome
Tat-mediated elongation of the HIV-1 transcript IEA Reactome
Tat-mediated elongation of the HIV-1 transcript TAS Reactome
Tat-mediated HIV elongation arrest and recovery IEA Reactome
TP53 Regulates Transcription of DNA Repair Genes TAS Reactome
Transcription of the HIV genome IEA Reactome
Transcription of the HIV genome TAS Reactome
Transcriptional Regulation by TP53 TAS Reactome
Viral Infection Pathways IEA Reactome
Viral Infection Pathways TAS Reactome





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