Gene description for NT5DC3
Gene name 5'-nucleotidase domain containing 3
Gene symbol NT5DC3
Other names/aliases TU12B1-TY
TU12B1TY
Species Homo sapiens
 Database cross references - NT5DC3
ExoCarta ExoCarta_51559
Vesiclepedia VP_51559
Entrez Gene 51559
HGNC 30826
MIM 611076
UniProt Q86UY8  
 NT5DC3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for NT5DC3
Molecular Function
    5'-nucleotidase activity GO:0008253 IBA
    metal ion binding GO:0046872 IEA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    cytosol GO:0005829 IEA
    receptor complex GO:0043235 IDA
 Experiment description of studies that identified NT5DC3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NT5DC3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 MAT2A 4144
Co-fractionation Homo sapiens
3 CLPP 8192
Proximity Label-MS Homo sapiens
4 VPS35 55737
Co-fractionation Homo sapiens
5 MARVELD2 153562
Affinity Capture-MS Homo sapiens
6 ZNF211  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 SERBP1 26135
Affinity Capture-MS Homo sapiens
8 FKBP10 60681
Co-fractionation Homo sapiens
9 XPO1 7514
Co-fractionation Homo sapiens
10 IL25  
Affinity Capture-MS Homo sapiens
11 RPA2 6118
Proximity Label-MS Homo sapiens
12 S100A2 6273
Co-fractionation Homo sapiens
13 RNMT 8731
Co-fractionation Homo sapiens
14 NDUFS7 374291
Affinity Capture-MS Homo sapiens
15 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
16 EPRS 2058
Co-fractionation Homo sapiens
17 NT5DC2 64943
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 DNAJC5 80331
Co-fractionation Homo sapiens
19 EXOSC2 23404
Affinity Capture-MS Homo sapiens
20 YARS2  
Affinity Capture-MS Homo sapiens
21 RXRA 6256
Co-fractionation Homo sapiens
22 PXN 5829
Co-fractionation Homo sapiens
23 ITGB4 3691
Co-fractionation Homo sapiens
24 KIF11 3832
Affinity Capture-MS Homo sapiens
25 ARHGEF6 9459
Affinity Capture-MS Homo sapiens
26 RPA3 6119
Proximity Label-MS Homo sapiens
27 Gsk3b  
Affinity Capture-MS Mus musculus
28 RCC1 1104
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which NT5DC3 is involved
No pathways found





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