Gene description for SDHAF2
Gene name succinate dehydrogenase complex assembly factor 2
Gene symbol SDHAF2
Other names/aliases C11orf79
PGL2
SDH5
Species Homo sapiens
 Database cross references - SDHAF2
ExoCarta ExoCarta_54949
Vesiclepedia VP_54949
Entrez Gene 54949
HGNC 26034
MIM 613019
UniProt Q9NX18  
 SDHAF2 identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for SDHAF2
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    tricarboxylic acid cycle GO:0006099 IBA
    mitochondrial electron transport, succinate to ubiquinone GO:0006121 IBA
    mitochondrial electron transport, succinate to ubiquinone GO:0006121 IMP
    protein dephosphorylation GO:0006470 IGI
    negative regulation of epithelial to mesenchymal transition GO:0010719 IMP
    protein-FAD linkage GO:0018293 IMP
    mitochondrial respiratory chain complex II assembly GO:0034553 IBA
    mitochondrial respiratory chain complex II assembly GO:0034553 IDA
    negative regulation of canonical Wnt signaling pathway GO:0090090 IGI
Subcellular Localization
    nucleolus GO:0005730 IDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrial matrix GO:0005759 IEA
    cytosol GO:0005829 IDA
 Experiment description of studies that identified SDHAF2 in sEVs
1
Experiment ID 234
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for SDHAF2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 IMMT 10989
Two-hybrid Homo sapiens
2 IKBKAP 8518
Two-hybrid Homo sapiens
3 CTNNA2 1496
Affinity Capture-MS Homo sapiens
4 UQCRFS1 7386
Co-fractionation Homo sapiens
5 OXCT1 5019
Co-fractionation Homo sapiens
6 SH3BGRL 6451
Co-fractionation Homo sapiens
7 EEF1A1 1915
Two-hybrid Homo sapiens
8 HSPA8 3312
Affinity Capture-MS Homo sapiens
9 SDHA 6389
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 PCMT1 5110
Co-fractionation Homo sapiens
11 SUCLG2 8801
Co-fractionation Homo sapiens
12 PLAUR 5329
Co-fractionation Homo sapiens
13 NOL8  
Affinity Capture-MS Homo sapiens
14 SLC1A5 6510
Affinity Capture-MS Homo sapiens
15 SKP2  
Affinity Capture-MS Homo sapiens
16 CTTN 2017
Co-fractionation Homo sapiens
17 KLHDC2  
Affinity Capture-MS Homo sapiens
18 RBM12 10137
Co-fractionation Homo sapiens
19 CYP4F2  
Two-hybrid Homo sapiens
20 CCDC90B  
Two-hybrid Homo sapiens
21 CWF19L2  
Affinity Capture-MS Homo sapiens
22 UBXN7 26043
Co-fractionation Homo sapiens
23 RRM2 6241
Affinity Capture-MS Homo sapiens
24 CCNB1 891
Co-fractionation Homo sapiens
25 CORO2A  
Affinity Capture-MS Homo sapiens
26 VCL 7414
Co-fractionation Homo sapiens
27 SEC22A  
Two-hybrid Homo sapiens
28 SUMO2 6613
Co-fractionation Homo sapiens
29 COA7  
Co-fractionation Homo sapiens
30 ABCA2 20
Affinity Capture-MS Homo sapiens
31 KRAS 3845
Negative Genetic Homo sapiens
32 HSPA9 3313
Affinity Capture-MS Homo sapiens
33 SSX2IP  
Affinity Capture-MS Homo sapiens
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