Gene description for Ddx21
Gene name DEAD (Asp-Glu-Ala-Asp) box polypeptide 21
Gene symbol Ddx21
Other names/aliases AI255159
AL022742
D10Ertd645e
D10Wsu42e
Species Mus musculus
 Database cross references - Ddx21
ExoCarta ExoCarta_56200
Vesiclepedia VP_56200
Entrez Gene 56200
UniProt Q9JIK5  
 Ddx21 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Ddx21
Molecular Function
    DNA clamp loader activity GO:0003689 IEA
    RNA binding GO:0003723 ISS
    RNA helicase activity GO:0003724 IBA
    RNA helicase activity GO:0003724 ISO
    RNA helicase activity GO:0003724 ISS
    double-stranded RNA binding GO:0003725 IDA
    mRNA binding GO:0003729 IBA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ATP hydrolysis activity GO:0016887 IEA
    rRNA binding GO:0019843 ISO
    rRNA binding GO:0019843 ISS
    snoRNA binding GO:0030515 ISO
    snoRNA binding GO:0030515 ISS
    miRNA binding GO:0035198 ISO
    miRNA binding GO:0035198 ISS
    identical protein binding GO:0042802 IDA
    cohesin loader activity GO:0061775 IEA
    7SK snRNA binding GO:0097322 ISO
    7SK snRNA binding GO:0097322 ISS
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
Biological Process
    positive regulation of myeloid dendritic cell cytokine production GO:0002735 IMP
    chromatin remodeling GO:0006338 NAS
    rRNA processing GO:0006364 IEA
    transcription by RNA polymerase II GO:0006366 ISO
    transcription by RNA polymerase II GO:0006366 ISS
    response to virus GO:0009615 IMP
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IMP
    response to exogenous dsRNA GO:0043330 IMP
    innate immune response GO:0045087 IEA
    positive regulation of transcription by RNA polymerase I GO:0045943 NAS
    positive regulation of transcription by RNA polymerase II GO:0045944 NAS
    positive regulation of transcription by RNA polymerase III GO:0045945 ISO
    defense response to virus GO:0051607 IEA
    R-loop processing GO:0062176 ISO
    R-loop processing GO:0062176 ISS
    chromatin looping GO:0140588 IEA
Subcellular Localization
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 ISO
    nucleoplasm GO:0005654 ISS
    chromosome GO:0005694 ISO
    nucleolus GO:0005730 IBA
    nucleolus GO:0005730 ISO
    nucleolus GO:0005730 ISS
    nucleolus GO:0005730 NAS
    mitochondrion GO:0005739 NAS
    cytosol GO:0005829 IDA
    B-WICH complex GO:0110016 ISO
 Experiment description of studies that identified Ddx21 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Ddx21
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DDX21 9188
Affinity Capture-MS Homo sapiens
2 Zbtb7b  
Affinity Capture-MS Mus musculus
3 KPNA6 23633
Affinity Capture-MS Homo sapiens
4 Fzr1  
Affinity Capture-MS Mus musculus
5 Atxn1  
Proximity Label-MS Mus musculus
Affinity Capture-MS Mus musculus
6 Eed  
Affinity Capture-MS Mus musculus
7 Tfe3  
Affinity Capture-MS Mus musculus
8 KPNA1 3836
Affinity Capture-MS Homo sapiens
9 Runx2  
Affinity Capture-MS Mus musculus
10 Dmrt2  
Affinity Capture-MS Mus musculus
11 Fancd2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here