Gene description for Arl3
Gene name ADP-ribosylation factor-like 3
Gene symbol Arl3
Other names/aliases -
Species Mus musculus
 Database cross references - Arl3
ExoCarta ExoCarta_56350
Vesiclepedia VP_56350
Entrez Gene 56350
UniProt Q9WUL7  
 Arl3 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Arl3
Molecular Function
    magnesium ion binding GO:0000287 IDA
    GTPase activity GO:0003924 IDA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 IDA
    GTP binding GO:0005525 ISO
    microtubule binding GO:0008017 ISO
    microtubule binding GO:0008017 ISS
    GDP binding GO:0019003 IDA
    GDP binding GO:0019003 ISO
Biological Process
    mitotic cytokinesis GO:0000281 ISO
    mitotic cytokinesis GO:0000281 ISS
    kidney development GO:0001822 IMP
    post-Golgi vesicle-mediated transport GO:0006892 ISO
    Golgi to plasma membrane transport GO:0006893 IMP
    smoothened signaling pathway GO:0007224 IMP
    small GTPase-mediated signal transduction GO:0007264 ISO
    small GTPase-mediated signal transduction GO:0007264 ISS
    protein transport GO:0015031 IEA
    intraciliary transport GO:0042073 IMP
    photoreceptor cell development GO:0042461 IMP
    cilium assembly GO:0060271 IBA
    cilium assembly GO:0060271 ISO
    cilium assembly GO:0060271 ISS
    protein localization to cilium GO:0061512 ISO
    protein localization to ciliary membrane GO:1903441 IMP
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    Golgi apparatus GO:0005794 ISO
    Golgi apparatus GO:0005794 ISS
    centrosome GO:0005813 ISO
    centrosome GO:0005813 ISS
    spindle microtubule GO:0005876 ISO
    spindle microtubule GO:0005876 ISS
    cytoplasmic microtubule GO:0005881 ISO
    cytoplasmic microtubule GO:0005881 ISS
    cilium GO:0005929 ISO
    cilium GO:0005929 ISS
    axoneme GO:0005930 IDA
    microtubule cytoskeleton GO:0015630 IBA
    midbody GO:0030496 ISO
    midbody GO:0030496 ISS
    photoreceptor connecting cilium GO:0032391 ISO
    photoreceptor connecting cilium GO:0032391 ISS
    ciliary transition zone GO:0035869 IDA
    ciliary basal body GO:0036064 IDA
 Experiment description of studies that identified Arl3 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Arl3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Pde6d  
Two-hybrid Mus musculus
2 PDE6D 5147
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml



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