Gene description for MCOLN1
Gene name mucolipin 1
Gene symbol MCOLN1
Other names/aliases MG-2
ML4
MLIV
MST080
MSTP080
TRP-ML1
TRPM-L1
TRPML1
Species Homo sapiens
 Database cross references - MCOLN1
ExoCarta ExoCarta_57192
Vesiclepedia VP_57192
Entrez Gene 57192
HGNC 13356
MIM 605248
UniProt Q9GZU1  
 MCOLN1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for MCOLN1
Molecular Function
    monoatomic anion channel activity GO:0005253 ISS
    monoatomic cation channel activity GO:0005261 NAS
    calcium channel activity GO:0005262 IDA
    calcium channel activity GO:0005262 TAS
    potassium channel activity GO:0005267 ISS
    sodium channel activity GO:0005272 ISS
    iron ion transmembrane transporter activity GO:0005381 IDA
    iron ion transmembrane transporter activity GO:0005381 TAS
    protein binding GO:0005515 IPI
    lipid binding GO:0008289 IEA
    identical protein binding GO:0042802 IPI
    NAADP-sensitive calcium-release channel activity GO:0072345 IBA
    intracellularly phosphatidylinositol-3,5-bisphosphate-gated monatomic cation channel activity GO:0097682 ISS
    ligand-gated calcium channel activity GO:0099604 IDA
Biological Process
    adaptive immune response GO:0002250 IEA
    monoatomic cation transport GO:0006812 NAS
    calcium-mediated signaling GO:0019722 IEA
    transferrin transport GO:0033572 TAS
    iron ion transmembrane transport GO:0034755 IMP
    sodium ion transmembrane transport GO:0035725 IEA
    release of sequestered calcium ion into cytosol GO:0051209 IEA
    protein homotetramerization GO:0051289 IDA
    calcium ion transmembrane transport GO:0070588 IDA
    calcium ion transmembrane transport GO:0070588 TAS
    cellular response to calcium ion GO:0071277 ISS
    cellular response to pH GO:0071467 ISS
    potassium ion transmembrane transport GO:0071805 IEA
    phagosome maturation GO:0090382 IDA
    autophagosome maturation GO:0097352 IEA
    monoatomic anion transmembrane transport GO:0098656 IEA
    calcium ion export GO:1901660 IDA
    positive regulation of lysosome organization GO:1905673 IDA
Subcellular Localization
    phagocytic cup GO:0001891 IEA
    nucleoplasm GO:0005654 IDA
    lysosome GO:0005764 IDA
    lysosomal membrane GO:0005765 HDA
    lysosomal membrane GO:0005765 IBA
    lysosomal membrane GO:0005765 IDA
    late endosome GO:0005770 IDA
    Golgi apparatus GO:0005794 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 NAS
    plasma membrane GO:0005886 TAS
    endosome membrane GO:0010008 TAS
    membrane GO:0016020 IDA
    membrane GO:0016020 NAS
    phagocytic vesicle membrane GO:0030670 IEA
    late endosome membrane GO:0031902 IEA
    cell projection GO:0042995 IEA
    intracellular membrane-bounded organelle GO:0043231 IDA
    receptor complex GO:0043235 IDA
 Experiment description of studies that identified MCOLN1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MCOLN1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TPST2 8459
Affinity Capture-MS Homo sapiens
2 LY6D 8581
Affinity Capture-MS Homo sapiens
3 MCOLN3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 MCOLN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 CCR6  
Affinity Capture-MS Homo sapiens
6 EDEM1  
Affinity Capture-MS Homo sapiens
7 CSGALNACT2 55454
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 SLC35E1 79939
Two-hybrid Homo sapiens
9 C6orf106  
Affinity Capture-MS Homo sapiens
10 VDAC2 7417
Co-fractionation Homo sapiens
11 SPPL2B 56928
Affinity Capture-MS Homo sapiens
12 CLEC2B  
Affinity Capture-MS Homo sapiens
13 TRIM27  
Two-hybrid Homo sapiens
14 GPR35  
Two-hybrid Homo sapiens
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