Gene description for SLC22A3
Gene name solute carrier family 22 (organic cation transporter), member 3
Gene symbol SLC22A3
Other names/aliases EMT
EMTH
OCT3
Species Homo sapiens
 Database cross references - SLC22A3
ExoCarta ExoCarta_6581
Vesiclepedia VP_6581
Entrez Gene 6581
HGNC 10967
MIM 604842
UniProt O75751  
 SLC22A3 identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for SLC22A3
Molecular Function
    neurotransmitter transmembrane transporter activity GO:0005326 IDA
    neurotransmitter transmembrane transporter activity GO:0005326 IMP
    protein binding GO:0005515 IPI
    monoamine transmembrane transporter activity GO:0008504 IDA
    monoamine transmembrane transporter activity GO:0008504 IMP
    organic anion transmembrane transporter activity GO:0008514 ISS
    organic cation transmembrane transporter activity GO:0015101 EXP
    organic cation transmembrane transporter activity GO:0015101 IBA
    organic cation transmembrane transporter activity GO:0015101 IDA
    organic cation transmembrane transporter activity GO:0015101 TAS
    spermidine transmembrane transporter activity GO:0015606 ISS
    quaternary ammonium group transmembrane transporter activity GO:0015651 IDA
    toxin transmembrane transporter activity GO:0019534 IDA
Biological Process
    histamine metabolic process GO:0001692 TAS
    monoatomic ion transport GO:0006811 IEA
    neurotransmitter transport GO:0006836 IDA
    serotonin transport GO:0006837 IDA
    organic cation transport GO:0015695 IBA
    organic cation transport GO:0015695 IDA
    organic cation transport GO:0015695 IDA
    quaternary ammonium group transport GO:0015697 IDA
    quaternary ammonium group transport GO:0015697 IDA
    organic anion transport GO:0015711 ISS
    monocarboxylic acid transport GO:0015718 IEA
    monoamine transport GO:0015844 IMP
    spermidine transport GO:0015848 ISS
    dopamine transport GO:0015872 IDA
    norepinephrine transport GO:0015874 IDA
    regulation of appetite GO:0032098 IEA
    xenobiotic transport GO:0042908 IDA
    epinephrine transport GO:0048241 IDA
    histamine transport GO:0051608 IDA
    serotonin uptake GO:0051610 IDA
    histamine uptake GO:0051615 IDA
    histamine uptake GO:0051615 IMP
    norepinephrine uptake GO:0051620 IDA
    epinephrine uptake GO:0051625 IDA
    purine-containing compound transmembrane transport GO:0072530 TAS
    dopamine uptake GO:0090494 IDA
    transport across blood-brain barrier GO:0150104 NAS
    spermidine transmembrane transport GO:1903711 IEA
    cellular detoxification GO:1990748 IDA
Subcellular Localization
    nuclear outer membrane GO:0005640 IEA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    endomembrane system GO:0012505 ISS
    membrane GO:0016020 TAS
    basolateral plasma membrane GO:0016323 IDA
    apical plasma membrane GO:0016324 IDA
    mitochondrial membrane GO:0031966 ISS
    neuronal cell body GO:0043025 IEA
    presynapse GO:0098793 IEA
 Experiment description of studies that identified SLC22A3 in sEVs
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for SLC22A3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PLXNB2 23654
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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