Gene description for SSR3
Gene name signal sequence receptor, gamma (translocon-associated protein gamma)
Gene symbol SSR3
Other names/aliases TRAPG
Species Homo sapiens
 Database cross references - SSR3
ExoCarta ExoCarta_6747
Vesiclepedia VP_6747
Entrez Gene 6747
HGNC 11325
MIM 606213
UniProt Q9UNL2  
 SSR3 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Endothelial cells 26027894    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for SSR3
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    SRP-dependent cotranslational protein targeting to membrane GO:0006614 IEA
Subcellular Localization
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum membrane GO:0005789 IEA
 Experiment description of studies that identified SSR3 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
6
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SSR3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPC 3183
Co-fractionation Homo sapiens
2 CLTC 1213
Co-fractionation Homo sapiens
3 SPRTN  
Affinity Capture-MS Homo sapiens
4 CLIP1 6249
Cross-Linking-MS (XL-MS) Homo sapiens
5 USP43  
Affinity Capture-MS Homo sapiens
6 SSR2  
Cross-Linking-MS (XL-MS) Homo sapiens
7 KIF20A 10112
Affinity Capture-MS Homo sapiens
8 RPL14 9045
Co-fractionation Homo sapiens
9 HSD17B12 51144
Co-fractionation Homo sapiens
10 GCLC 2729
Cross-Linking-MS (XL-MS) Homo sapiens
11 TOMM22 56993
Co-fractionation Homo sapiens
12 KIAA1429 25962
Affinity Capture-MS Homo sapiens
13 CAPZB 832
Affinity Capture-MS Homo sapiens
14 TOP2A 7153
Affinity Capture-MS Homo sapiens
15 RPS20 6224
Cross-Linking-MS (XL-MS) Homo sapiens
16 RBM39 9584
Affinity Capture-MS Homo sapiens
17 SF3B1 23451
Co-fractionation Homo sapiens
18 FBXW7  
Affinity Capture-MS Homo sapiens
19 PRC1 9055
Affinity Capture-MS Homo sapiens
20 SSR1 6745
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
21 DDOST 1650
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
22 RPN2 6185
Affinity Capture-MS Homo sapiens
23 C20orf24  
Affinity Capture-MS Homo sapiens
24 CHORDC1 26973
Affinity Capture-MS Homo sapiens
25 CCDC47 57003
Affinity Capture-MS Homo sapiens
26 PHB 5245
Co-fractionation Homo sapiens
27 SEPT7 989
Co-fractionation Homo sapiens
28 ZWINT  
Affinity Capture-MS Homo sapiens
29 RBM42  
Affinity Capture-MS Homo sapiens
30 RBM8A 9939
Affinity Capture-MS Homo sapiens
31 KIF14 9928
Affinity Capture-MS Homo sapiens
32 HMGCS2 3158
Affinity Capture-MS Homo sapiens
33 CHMP4C 92421
Affinity Capture-MS Homo sapiens
34 SRP9 6726
Affinity Capture-MS Homo sapiens
35 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
36 DDX21 9188
Co-fractionation Homo sapiens
37 RB1CC1 9821
Affinity Capture-MS Homo sapiens
38 Rock1  
Affinity Capture-MS Mus musculus
39 Cep44  
Affinity Capture-MS Mus musculus
40 DRG1 4733
Affinity Capture-MS Homo sapiens
41 TCEAL1  
Affinity Capture-MS Homo sapiens
42 BRE 9577
Affinity Capture-MS Homo sapiens
43 MAPRE1 22919
Affinity Capture-MS Homo sapiens
44 FAM162A 26355
Co-fractionation Homo sapiens
45 Ppp2ca 19052
Affinity Capture-MS Mus musculus
46 RPL5 6125
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
47 RPN1 6184
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
48 OST4  
Affinity Capture-MS Homo sapiens
49 DDX39B 7919
Co-fractionation Homo sapiens
50 SND1 27044
Co-fractionation Homo sapiens
51 SRP68 6730
Affinity Capture-MS Homo sapiens
52 VDAC2 7417
Co-fractionation Homo sapiens
53 SEC61B 10952
Affinity Capture-MS Homo sapiens
54 COX4I1 1327
Co-fractionation Homo sapiens
55 AQP2 359
Two-hybrid Homo sapiens
56 RPL10A 4736
Co-fractionation Homo sapiens
57 RPLP2 6181
Co-fractionation Homo sapiens
58 FANCD2  
Affinity Capture-MS Homo sapiens
59 CANX 821
Affinity Capture-MS Homo sapiens
60 UQCRC2 7385
Co-fractionation Homo sapiens
61 PCDHB15  
Affinity Capture-MS Homo sapiens
62 MYH14 79784
Affinity Capture-MS Homo sapiens
63 ASPM 259266
Affinity Capture-MS Homo sapiens
64 KLHL29  
Affinity Capture-MS Homo sapiens
65 YIPF5 81555
Affinity Capture-MS Homo sapiens
66 PCBP4 57060
Affinity Capture-MS Homo sapiens
67 PSPC1 55269
Affinity Capture-MS Homo sapiens
68 RPA3 6119
Proximity Label-MS Homo sapiens
69 TMCO1 54499
Co-fractionation Homo sapiens
70 Tubg1 103733
Affinity Capture-MS Mus musculus
71 NHP2L1 4809
Co-fractionation Homo sapiens
72 FAM169A  
Cross-Linking-MS (XL-MS) Homo sapiens
73 SF3B3 23450
Co-fractionation Homo sapiens
74 VDAC1 7416
Co-fractionation Homo sapiens
75 XRCC3  
Affinity Capture-MS Homo sapiens
76 Slc6a8 102857
Affinity Capture-MS Mus musculus
77 RTN4 57142
Co-fractionation Homo sapiens
78 MAGT1 84061
Co-fractionation Homo sapiens
79 RPS16 6217
Affinity Capture-MS Homo sapiens
80 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
81 PRRC2A 7916
Two-hybrid Homo sapiens
82 PDE4B  
Protein-RNA Homo sapiens
83 ATP5L 10632
Co-fractionation Homo sapiens
84 PGRMC1 10857
Affinity Capture-MS Homo sapiens
85 ERLIN1 10613
Co-fractionation Homo sapiens
86 Dync1li1 235661
Affinity Capture-MS Mus musculus
87 Itgb1 16412
Affinity Capture-MS Mus musculus
88 SAP18 10284
Co-fractionation Homo sapiens
89 SRP19 6728
Affinity Capture-MS Homo sapiens
90 NGEF  
Affinity Capture-MS Homo sapiens
91 PRMT7  
Affinity Capture-MS Homo sapiens
92 TRAT1 50852
Affinity Capture-MS Homo sapiens
93 SRP72 6731
Affinity Capture-MS Homo sapiens
94 HSP90AA1 3320
Co-fractionation Homo sapiens
95 DDX39A 10212
Co-fractionation Homo sapiens
96 TRIM43B  
Cross-Linking-MS (XL-MS) Homo sapiens
97 EPHA2 1969
Affinity Capture-MS Homo sapiens
98 LDLR 3949
Negative Genetic Homo sapiens
99 GNB2L1 10399
Affinity Capture-MS Homo sapiens
100 RABAC1 10567
Two-hybrid Homo sapiens
101 ARHGEF10L 55160
Affinity Capture-MS Homo sapiens
102 TRIM43  
Cross-Linking-MS (XL-MS) Homo sapiens
103 VDAC3 7419
Co-fractionation Homo sapiens
104 SEC61A1 29927
Co-fractionation Homo sapiens
105 CAPN11  
Cross-Linking-MS (XL-MS) Homo sapiens
View the network image/svg+xml
 Pathways in which SSR3 is involved
PathwayEvidenceSource
Metabolism of proteins IEA Reactome
SRP-dependent cotranslational protein targeting to membrane IEA Reactome
Translation IEA Reactome





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