Gene description for Cad
Gene name carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
Gene symbol Cad
Other names/aliases 2410008J01Rik
AU018859
Cpad
Species Mus musculus
 Database cross references - Cad
ExoCarta ExoCarta_69719
Vesiclepedia VP_69719
Entrez Gene 69719
UniProt B2RQC6  
 Cad identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Cad
Molecular Function
    UTP binding GO:0002134 ISO
    aspartate carbamoyltransferase activity GO:0004070 IBA
    aspartate carbamoyltransferase activity GO:0004070 IDA
    aspartate carbamoyltransferase activity GO:0004070 ISO
    carbamoyl-phosphate synthase (ammonia) activity GO:0004087 IEA
    carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity GO:0004088 IDA
    carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity GO:0004088 IMP
    carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity GO:0004088 ISO
    dihydroorotase activity GO:0004151 IBA
    dihydroorotase activity GO:0004151 IMP
    dihydroorotase activity GO:0004151 ISO
    dihydroorotase activity GO:0004151 ISS
    glutaminase activity GO:0004359 IEA
    protein kinase activity GO:0004672 ISO
    ATP binding GO:0005524 ISO
    zinc ion binding GO:0008270 ISO
    zinc ion binding GO:0008270 ISS
    amino acid binding GO:0016597 IEA
    enzyme binding GO:0019899 IEA
    enzyme binding GO:0019899 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
Biological Process
    liver development GO:0001889 IEA
    'de novo' pyrimidine nucleobase biosynthetic process GO:0006207 IBA
    'de novo' pyrimidine nucleobase biosynthetic process GO:0006207 ISO
    'de novo' pyrimidine nucleobase biosynthetic process GO:0006207 ISO
    'de novo' pyrimidine nucleobase biosynthetic process GO:0006207 ISS
    UDP biosynthetic process GO:0006225 ISO
    UTP biosynthetic process GO:0006228 IEA
    UTP biosynthetic process GO:0006228 ISO
    glutamine metabolic process GO:0006541 IBA
    glutamine metabolic process GO:0006541 IEA
    glutamine metabolic process GO:0006541 ISO
    xenobiotic metabolic process GO:0006805 IEA
    xenobiotic metabolic process GO:0006805 ISO
    heart development GO:0007507 IEA
    female pregnancy GO:0007565 IEA
    lactation GO:0007595 IEA
    response to amine GO:0014075 IEA
    peptidyl-threonine phosphorylation GO:0018107 ISO
    citrulline biosynthetic process GO:0019240 IEA
    citrulline biosynthetic process GO:0019240 ISO
    response to caffeine GO:0031000 IEA
    animal organ regeneration GO:0031100 IEA
    response to insulin GO:0032868 IEA
    response to insulin GO:0032868 ISO
    response to testosterone GO:0033574 IEA
    response to starvation GO:0042594 IEA
    response to starvation GO:0042594 ISO
    'de novo' UMP biosynthetic process GO:0044205 IEA
    'de novo' UMP biosynthetic process GO:0044205 IMP
    'de novo' UMP biosynthetic process GO:0044205 ISO
    protein autophosphorylation GO:0046777 ISO
    response to cortisol GO:0051414 IEA
    cellular response to epidermal growth factor stimulus GO:0071364 IEA
Subcellular Localization
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
    nuclear matrix GO:0016363 IEA
    nuclear matrix GO:0016363 ISO
    protein-containing complex GO:0032991 IEA
    protein-containing complex GO:0032991 ISO
    cell projection GO:0042995 ISO
    neuronal cell body GO:0043025 IEA
    neuronal cell body GO:0043025 ISO
    terminal bouton GO:0043195 IEA
    terminal bouton GO:0043195 ISO
 Experiment description of studies that identified Cad in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 214
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 215
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 216
MISEV standards
✔
EM
Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors "Hassani K, Olivier M."
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Cad
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Fxr1 14359
Affinity Capture-MS Mus musculus
2 Chaf1a  
Affinity Capture-MS Mus musculus
3 Tsc1  
Affinity Capture-MS Mus musculus
4 Pde4dip  
Affinity Capture-MS Mus musculus
5 Foxp3  
Affinity Capture-MS Mus musculus
6 Kctd13  
Affinity Capture-MS Mus musculus
7 Mycbp2 105689
Affinity Capture-MS Mus musculus
8 Fitm2  
Positive Genetic Mus musculus
9 Nphp1  
Affinity Capture-MS Mus musculus
10 Pou5f1  
Affinity Capture-MS Mus musculus
11 Atg12  
Positive Genetic Mus musculus
12 Iqcb1  
Affinity Capture-MS Mus musculus
13 Fancd2  
Affinity Capture-MS Mus musculus
14 Tnik  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which Cad is involved
PathwayEvidenceSource
Metabolism IEA Reactome
Metabolism of nucleotides IEA Reactome
Nucleotide biosynthesis IEA Reactome
Pyrimidine biosynthesis IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here