Gene description for Pdia6
Gene name protein disulfide isomerase associated 6
Gene symbol Pdia6
Other names/aliases 1700015E05Rik
AL023058
C77895
CaBP5
P5
Txndc7
Species Mus musculus
 Database cross references - Pdia6
ExoCarta ExoCarta_71853
Vesiclepedia VP_71853
Entrez Gene 71853
UniProt Q922R8  
 Pdia6 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Pdia6
Molecular Function
    protein disulfide isomerase activity GO:0003756 ISS
    protein-disulfide reductase activity GO:0015035 IBA
    protein-disulfide reductase activity GO:0015035 IMP
    protein-disulfide reductase activity GO:0015035 ISO
Biological Process
    platelet activation GO:0030168 ISS
    response to endoplasmic reticulum stress GO:0034976 IBA
    platelet aggregation GO:0070527 ISS
    negative regulation of IRE1-mediated unfolded protein response GO:1903895 IMP
Subcellular Localization
    extracellular space GO:0005615 ISO
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum lumen GO:0005788 IEA
    endoplasmic reticulum lumen GO:0005788 ISO
    smooth endoplasmic reticulum GO:0005790 ISO
    endoplasmic reticulum-Golgi intermediate compartment GO:0005793 ISO
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 ISS
    endoplasmic reticulum chaperone complex GO:0034663 IDA
    melanosome GO:0042470 ISS
 Experiment description of studies that identified Pdia6 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Pdia6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Pdia3 14827
Co-fractionation Mus musculus
2 Mettl21e  
Affinity Capture-MS Mus musculus
3 Tmem173  
Proximity Label-MS Mus musculus
4 App 11820
Affinity Capture-MS Mus musculus
5 Sdf2l1  
Co-fractionation Mus musculus
6 Eed  
Affinity Capture-MS Mus musculus
7 Tfe3  
Affinity Capture-MS Mus musculus
8 Tpd52l2  
Co-fractionation Mus musculus
9 Unk  
Affinity Capture-RNA Mus musculus
10 Fancd2  
Affinity Capture-MS Mus musculus
11 Kctd13  
Affinity Capture-MS Mus musculus
12 Lipc  
Affinity Capture-MS Mus musculus
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