Gene description for Rhoh
Gene name ras homolog gene family, member H
Gene symbol Rhoh
Other names/aliases 5830400A04Rik
AU019774
Arhh
Species Mus musculus
 Database cross references - Rhoh
ExoCarta ExoCarta_74734
Vesiclepedia VP_74734
Entrez Gene 74734
UniProt Q9D3G9  
 Rhoh identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rhoh
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IEA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 IEA
    GTP binding GO:0005525 ISO
    kinase inhibitor activity GO:0019210 IEA
    kinase inhibitor activity GO:0019210 ISO
    protein kinase binding GO:0019901 IBA
Biological Process
    actin filament organization GO:0007015 IBA
    signal transduction GO:0007165 IBA
    small GTPase-mediated signal transduction GO:0007264 IEA
    T cell differentiation GO:0030217 IDA
    negative regulation of canonical NF-kappaB signal transduction GO:0043124 IEA
    negative regulation of canonical NF-kappaB signal transduction GO:0043124 ISO
    mast cell activation GO:0045576 IDA
    positive regulation of T cell differentiation GO:0045582 IEA
    positive regulation of T cell differentiation GO:0045582 ISO
Subcellular Localization
    immunological synapse GO:0001772 IDA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
 Experiment description of studies that identified Rhoh in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rhoh
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TGFBR1 7046
Affinity Capture-Luminescence Homo sapiens
2 Usp7  
Affinity Capture-MS Mus musculus
View the network image/svg+xml



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