Gene description for EEPD1
Gene name endonuclease/exonuclease/phosphatase family domain containing 1
Gene symbol EEPD1
Other names/aliases HSPC107
Species Homo sapiens
 Database cross references - EEPD1
ExoCarta ExoCarta_80820
Vesiclepedia VP_80820
Entrez Gene 80820
HGNC 22223
UniProt Q7L9B9  
 EEPD1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for EEPD1
Molecular Function
    DNA binding GO:0003677 IEA
    catalytic activity GO:0003824 IEA
    protein binding GO:0005515 IPI
Biological Process
    DNA repair GO:0006281 IEA
    positive regulation of cholesterol efflux GO:0010875 IMP
Subcellular Localization
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IMP
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified EEPD1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for EEPD1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SDC1 6382
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 FEM1A  
Affinity Capture-MS Homo sapiens
3 NCSTN 23385
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 SPATA7 55812
Affinity Capture-MS Homo sapiens
5 CIAO1 9391
Affinity Capture-MS Homo sapiens
6 FSD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 PCDHGC3 5098
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 RSBN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 APP 351
Reconstituted Complex Homo sapiens
10 ANKRD50 57182
Affinity Capture-MS Homo sapiens
11 STARD6  
Affinity Capture-MS Homo sapiens
12 CERS2 29956
Affinity Capture-MS Homo sapiens
13 GJB7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 MED21  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 TIGD5  
Affinity Capture-MS Homo sapiens
16 PIGT 51604
Affinity Capture-MS Homo sapiens
17 GAGE5  
Affinity Capture-MS Homo sapiens
18 EPHA2 1969
Proximity Label-MS Homo sapiens
19 ISG20  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 DPM2 8818
Affinity Capture-MS Homo sapiens
21 KRAS 3845
Proximity Label-MS Homo sapiens
22 RHOB 388
Proximity Label-MS Homo sapiens
23 FYTTD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 GRIP1  
Two-hybrid Homo sapiens
25 MALL  
Affinity Capture-MS Homo sapiens
26 TMEM185A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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