Gene description for WNT5B
Gene name wingless-type MMTV integration site family, member 5B
Gene symbol WNT5B
Other names/aliases -
Species Homo sapiens
 Database cross references - WNT5B
ExoCarta ExoCarta_81029
Vesiclepedia VP_81029
Entrez Gene 81029
HGNC 16265
MIM 606361
UniProt Q9H1J7  
 WNT5B identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Urine 19056867    
 Gene ontology annotations for WNT5B
Molecular Function
    signaling receptor binding GO:0005102 IPI
    frizzled binding GO:0005109 IBA
    cytokine activity GO:0005125 IBA
Biological Process
    chondrocyte differentiation GO:0002062 IEP
    neuron differentiation GO:0030182 IBA
    neuron differentiation GO:0030182 ISS
    positive regulation of cell migration GO:0030335 IDA
    muscle cell differentiation GO:0042692 ISS
    cell fate commitment GO:0045165 IBA
    fat cell differentiation GO:0045444 NAS
    canonical Wnt signaling pathway GO:0060070 IBA
    lens fiber cell development GO:0070307 ISS
    cellular response to retinoic acid GO:0071300 ISS
    positive regulation of convergent extension involved in gastrulation GO:1904105 ISS
    positive regulation of non-canonical Wnt signaling pathway GO:2000052 ISS
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IBA
    extracellular space GO:0005615 NAS
    endoplasmic reticulum lumen GO:0005788 TAS
    Golgi lumen GO:0005796 TAS
    plasma membrane GO:0005886 TAS
    endocytic vesicle membrane GO:0030666 TAS
    collagen-containing extracellular matrix GO:0062023 HDA
    extracellular exosome GO:0070062 HDA
    extracellular exosome GO:0070062 TAS
 Experiment description of studies that identified WNT5B in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for WNT5B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KLRG2 346689
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 APPBP2  
Two-hybrid Homo sapiens
3 WNT5A 7474
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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