Gene description for Tceb2
Gene name transcription elongation factor B (SIII), polypeptide 2
Gene symbol Tceb2
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Tceb2
ExoCarta ExoCarta_81807
Vesiclepedia VP_81807
Entrez Gene 81807
UniProt P62870  
 Tceb2 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Tceb2
Molecular Function
    transcription corepressor binding GO:0001222 ISO
    transcription coactivator activity GO:0003713 IDA
    ubiquitin-protein transferase activity GO:0004842 IC
    ubiquitin protein ligase binding GO:0031625 ISO
    protein-containing complex binding GO:0044877 IPI
Biological Process
    transcription initiation at RNA polymerase II promoter GO:0006367 ISO
    transcription elongation by RNA polymerase II GO:0006368 IEA
    protein ubiquitination GO:0016567 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    target-directed miRNA degradation GO:0140958 ISO
Subcellular Localization
    transcription regulator complex GO:0005667 IDA
    VCB complex GO:0030891 IBA
    VCB complex GO:0030891 IDA
    VCB complex GO:0030891 IEA
    Cul2-RING ubiquitin ligase complex GO:0031462 ISO
    Cul5-RING ubiquitin ligase complex GO:0031466 ISO
    elongin complex GO:0070449 IBA
    elongin complex GO:0070449 IEA
    elongin complex GO:0070449 ISO
 Experiment description of studies that identified Tceb2 in sEVs
1
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Tceb2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Lrrc41  
Co-fractionation Rattus norvegicus
2 Tceb1 64525
Co-fractionation Rattus norvegicus
Reconstituted Complex Rattus norvegicus
3 CUL5 8065
Affinity Capture-Western Homo sapiens
4 CUL3 8452
Affinity Capture-Western Homo sapiens
5 Mib1  
Affinity Capture-MS Rattus norvegicus
6 Cul4a  
Affinity Capture-Western Mus musculus
7 VHL  
Reconstituted Complex Homo sapiens
8 Tceb3  
Affinity Capture-Western Rattus norvegicus
Reconstituted Complex Rattus norvegicus
View the network image/svg+xml



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