Gene description for LARGE
Gene name like-glycosyltransferase
Gene symbol LARGE
Other names/aliases MDC1D
MDDGA6
MDDGB6
Species Homo sapiens
 Database cross references - LARGE
ExoCarta ExoCarta_9215
Vesiclepedia VP_9215
Entrez Gene 9215
HGNC 6511
MIM 603590
UniProt O95461  
 LARGE identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for LARGE
Molecular Function
    protein binding GO:0005515 IPI
    acetylglucosaminyltransferase activity GO:0008375 TAS
    glucuronosyltransferase activity GO:0015020 IBA
    glucuronosyltransferase activity GO:0015020 IDA
    glucuronosyltransferase activity GO:0015020 TAS
    glycosyltransferase activity GO:0016757 TAS
    hexosyltransferase activity GO:0016758 IDA
    manganese ion binding GO:0030145 IDA
    UDP-xylosyltransferase activity GO:0035252 TAS
    xylosyltransferase activity GO:0042285 IBA
    xylosyltransferase activity GO:0042285 IDA
Biological Process
    blood vessel development GO:0001568 IEA
    behavioral fear response GO:0001662 IEA
    neuron migration GO:0001764 IEA
    N-acetylglucosamine metabolic process GO:0006044 TAS
    protein glycosylation GO:0006486 IMP
    protein O-linked glycosylation GO:0006493 TAS
    protein targeting to membrane GO:0006612 IEA
    glycosphingolipid biosynthetic process GO:0006688 TAS
    water transport GO:0006833 IEA
    intracellular protein transport GO:0006886 IEA
    striated muscle contraction GO:0006941 IEA
    plasma membrane organization GO:0007009 IEA
    cytoskeleton organization GO:0007010 IEA
    neuromuscular synaptic transmission GO:0007274 IEA
    sensory perception of sound GO:0007605 IEA
    memory GO:0007613 IEA
    determination of adult lifespan GO:0008340 IEA
    glycoprotein biosynthetic process GO:0009101 TAS
    response to light stimulus GO:0009416 IEA
    response to mechanical stimulus GO:0009612 IEA
    gene expression GO:0010467 IEA
    retina layer formation GO:0010842 IEA
    dentate gyrus development GO:0021542 IEA
    nerve development GO:0021675 IEA
    principal sensory nucleus of trigeminal nerve development GO:0021740 IEA
    macrophage differentiation GO:0030225 IEA
    post-embryonic hindlimb morphogenesis GO:0035129 IEA
    multicellular organism growth GO:0035264 IEA
    protein O-linked mannosylation GO:0035269 IBA
    protein O-linked mannosylation GO:0035269 IDA
    myelination GO:0042552 IEA
    skeletal muscle tissue regeneration GO:0043403 ISS
    post-translational protein modification GO:0043687 IEA
    muscle cell cellular homeostasis GO:0046716 ISS
    astrocyte differentiation GO:0048708 IEA
    skeletal muscle fiber development GO:0048741 IEA
    neuromuscular process controlling posture GO:0050884 IEA
    synaptic assembly at neuromuscular junction GO:0051124 IEA
    localization of cell GO:0051674 IEA
    cardiac muscle cell development GO:0055013 IEA
    long-term synaptic potentiation GO:0060291 IEA
    bone development GO:0060348 IEA
    skeletal muscle organ development GO:0060538 ISS
    retina vasculature development in camera-type eye GO:0061298 IEA
    connective tissue development GO:0061448 IEA
    protein-containing complex assembly GO:0065003 IEA
    basement membrane organization GO:0071711 IEA
    potassium ion transmembrane transport GO:0071805 IEA
    protein localization to plasma membrane GO:0072659 IEA
    walking behavior GO:0090659 IEA
    acetylcholine receptor signaling pathway GO:0095500 IEA
    skeletal muscle fiber differentiation GO:0098528 IEA
    reactive gliosis GO:0150103 IEA
Subcellular Localization
    Golgi membrane GO:0000139 TAS
    Golgi apparatus GO:0005794 IBA
    Golgi apparatus GO:0005794 IDA
    plasma membrane GO:0005886 IEA
    neuromuscular junction GO:0031594 IEA
    protein-containing complex GO:0032991 IEA
 Experiment description of studies that identified LARGE in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for LARGE
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CCL3  
Affinity Capture-MS Homo sapiens
2 GPIHBP1  
Affinity Capture-MS Homo sapiens
3 CEACAM8 1088
Affinity Capture-MS Homo sapiens
4 SCGB2A2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 TMEM25  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 PLAUR 5329
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 PDGFRA 5156
Affinity Capture-MS Homo sapiens
8 RLN1  
Affinity Capture-MS Homo sapiens
9 GAA 2548
Affinity Capture-MS Homo sapiens
10 SLAMF1 6504
Affinity Capture-MS Homo sapiens
11 CLEC12B  
Affinity Capture-MS Homo sapiens
12 HLA-DQA1 3117
Affinity Capture-MS Homo sapiens
13 SLC3A2 6520
Affinity Capture-MS Homo sapiens
14 RANBP10 57610
Affinity Capture-MS Homo sapiens
15 C1orf54  
Affinity Capture-MS Homo sapiens
16 PSCA 8000
Affinity Capture-MS Homo sapiens
17 SCGB2A1 4246
Affinity Capture-MS Homo sapiens
18 HYOU1 10525
Affinity Capture-MS Homo sapiens
19 FAM19A2 338811
Affinity Capture-MS Homo sapiens
20 KLRC1  
Affinity Capture-MS Homo sapiens
21 BAGE2  
Affinity Capture-MS Homo sapiens
22 CCDC8  
Affinity Capture-MS Homo sapiens
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