Gene description for PIEZO1
Gene name piezo-type mechanosensitive ion channel component 1
Gene symbol PIEZO1
Other names/aliases DHS
FAM38A
Mib
Species Homo sapiens
 Database cross references - PIEZO1
ExoCarta ExoCarta_9780
Vesiclepedia VP_9780
Entrez Gene 9780
HGNC 28993
MIM 611184
UniProt Q92508  
 PIEZO1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for PIEZO1
Molecular Function
    monoatomic cation channel activity GO:0005261 IBA
    monoatomic cation channel activity GO:0005261 ISS
    protein binding GO:0005515 IPI
    mechanosensitive monoatomic ion channel activity GO:0008381 IBA
    mechanosensitive monoatomic cation channel activity GO:0140135 IDA
Biological Process
    monoatomic cation transport GO:0006812 ISS
    positive regulation of myotube differentiation GO:0010831 IMP
    positive regulation of integrin activation GO:0033625 IMP
    positive regulation of cell-cell adhesion mediated by integrin GO:0033634 IMP
    regulation of membrane potential GO:0042391 IBA
    detection of mechanical stimulus GO:0050982 IBA
    cellular response to mechanical stimulus GO:0071260 IBA
    monoatomic cation transmembrane transport GO:0098655 IEA
Subcellular Localization
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum membrane GO:0005789 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISS
    lamellipodium membrane GO:0031258 IEA
    stereocilium GO:0032420 ISS
    cuticular plate GO:0032437 ISS
    endoplasmic reticulum-Golgi intermediate compartment membrane GO:0033116 IEA
 Experiment description of studies that identified PIEZO1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for PIEZO1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 IFNGR2  
Affinity Capture-MS Homo sapiens
2 Rab5c 19345
Affinity Capture-MS Mus musculus
3 NPC2 10577
Affinity Capture-MS Homo sapiens
4 GJB2  
Affinity Capture-MS Homo sapiens
5 TSPAN5 10098
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 OR10H1  
Affinity Capture-MS Homo sapiens
7 CLGN 1047
Affinity Capture-MS Homo sapiens
8 CXCR4 7852
Affinity Capture-MS Homo sapiens
9 TTYH1  
Affinity Capture-MS Homo sapiens
10 RAB7A 7879
Affinity Capture-MS Homo sapiens
11 VAPA 9218
Affinity Capture-MS Homo sapiens
12 SLC39A4 55630
Affinity Capture-MS Homo sapiens
13 KCNK1  
Affinity Capture-MS Homo sapiens
14 LGALS3 3958
Affinity Capture-MS Homo sapiens
15 GOLT1B 51026
Affinity Capture-MS Homo sapiens
16 CRELD1 78987
Affinity Capture-MS Homo sapiens
17 TSPAN15 23555
Affinity Capture-MS Homo sapiens
18 LGALS1 3956
Affinity Capture-MS Homo sapiens
19 CLEC12B  
Affinity Capture-MS Homo sapiens
20 LGALS9 3965
Affinity Capture-MS Homo sapiens
21 SORD 6652
Co-fractionation Homo sapiens
22 DNAJC5 80331
Proximity Label-MS Homo sapiens
23 TSPAN2 10100
Affinity Capture-MS Homo sapiens
24 CYP20A1 57404
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 MS4A15  
Affinity Capture-MS Homo sapiens
26 CHRNA5  
Affinity Capture-MS Homo sapiens
27 DNAJC5B  
Proximity Label-MS Homo sapiens
28 FBXO2 26232
Affinity Capture-MS Homo sapiens
29 CMTM5  
Affinity Capture-MS Homo sapiens
30 DSTN 11034
Co-fractionation Homo sapiens
31 DMRT1  
Affinity Capture-MS Homo sapiens
32 RAB5C 5878
Affinity Capture-MS Homo sapiens
33 GPR55  
Affinity Capture-MS Homo sapiens
34 YIPF3 25844
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 CHRNA4  
Affinity Capture-MS Homo sapiens
36 ATP2A3 489
Affinity Capture-MS Homo sapiens
37 C1orf95  
Affinity Capture-MS Homo sapiens
38 FPR2  
Affinity Capture-MS Homo sapiens
39 C3orf52  
Affinity Capture-MS Homo sapiens
40 ATP2A1 487
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here