Gene description for Mapre1
Gene name microtubule-associated protein, RP/EB family, member 1
Gene symbol Mapre1
Other names/aliases Eb1
Species Rattus norvegicus
 Database cross references - Mapre1
ExoCarta ExoCarta_114764
Entrez Gene 114764
UniProt Q66HR2  
 Mapre1 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Mapre1
Molecular Function
    poly(A) RNA binding GO:0044822 ISO
    microtubule plus-end binding GO:0051010 ISO
Biological Process
    positive regulation of microtubule plus-end binding GO:1903033 ISO
    protein localization to microtubule GO:0035372 ISS
    mitotic nuclear division GO:0007067 IEA
    cell division GO:0051301 IEA
    regulation of microtubule plus-end binding GO:1903031 IEA
    negative regulation of microtubule polymerization GO:0031115 ISO
Subcellular Localization
    microtubule GO:0005874 ISS
    cytoplasm GO:0005737 ISO
    cytoplasmic microtubule GO:0005881 ISO
    Golgi apparatus GO:0005794 ISO
    cell projection GO:0042995 ISO
    microtubule organizing center GO:0005815 IEA
    centrosome GO:0005813 ISO
    cortical microtubule cytoskeleton GO:0030981 ISO
    microtubule cytoskeleton GO:0015630 ISO
    spindle GO:0005819 ISO
    microtubule plus-end GO:0035371 ISO
    cell projection membrane GO:0031253 ISO
 Experiment description of studies that identified Mapre1 in exosomes
1
Experiment ID 95
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Mapre1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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