Gene description for Ppat
Gene name phosphoribosyl pyrophosphate amidotransferase
Gene symbol Ppat
Other names/aliases Atase
Species Rattus norvegicus
 Database cross references - Ppat
ExoCarta ExoCarta_117544
Entrez Gene 117544
UniProt P35433  
 Ppat identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Ppat
Molecular Function
    amidophosphoribosyltransferase activity GO:0004044 IDA
    metal ion binding GO:0046872 IEA
    4 iron, 4 sulfur cluster binding GO:0051539 IEA
Biological Process
    organ regeneration GO:0031100 IEP
    'de novo' IMP biosynthetic process GO:0006189 IEA
    response to drug GO:0042493 IEP
    purine nucleobase biosynthetic process GO:0009113 IEA
    cellular response to insulin stimulus GO:0032869 IEP
    G1/S transition of mitotic cell cycle GO:0000082 IEP
    ribose phosphate metabolic process GO:0019693 IDA
    maternal process involved in female pregnancy GO:0060135 IEP
    lactation GO:0007595 IEP
    cellular response to drug GO:0035690 IEP
    protein homotetramerization GO:0051289 IDA
    purine ribonucleoside monophosphate biosynthetic process GO:0009168 IDA
    glutamine catabolic process GO:0006543 IDA
    nucleoside metabolic process GO:0009116 IEA
    kidney development GO:0001822 IEP
 Experiment description of studies that identified Ppat in exosomes
1
Experiment ID 95
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Ppat
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Ppat is involved
PathwayEvidenceSource
Purine ribonucleoside monophosphate biosynthesis IEA Reactome





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