Gene description for C5ar1
Gene name complement component 5a receptor 1
Gene symbol C5ar1
Other names/aliases C5aR
C5r1
Cd88
D7Msu1
Species Mus musculus
 Database cross references - C5ar1
ExoCarta ExoCarta_12273
Entrez Gene 12273
UniProt P30993  
 C5ar1 identified in exosomes derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for C5ar1
Molecular Function
    G-protein coupled receptor activity GO:0004930 IEA
    anaphylatoxin receptor activity GO:0004942 IEA
    C5a anaphylatoxin receptor activity GO:0004944 ISO
    complement component C5a receptor activity GO:0004878 ISO
    signal transducer activity GO:0004871 IEA
    complement component C5a binding GO:0001856 ISO
Biological Process
    G-protein coupled receptor signaling pathway GO:0007186 IDA
    positive regulation of angiogenesis GO:0045766 IMP
    mRNA transcription from RNA polymerase II promoter GO:0042789 ISO
    complement receptor mediated signaling pathway GO:0002430 ISO
    cell proliferation in hindbrain GO:0021534 ISO
    response to peptidoglycan GO:0032494 IMP
    positive regulation of epithelial cell proliferation GO:0050679 ISO
    positive regulation of vascular endothelial growth factor production GO:0010575 IMP
    positive regulation of macrophage chemotaxis GO:0010759 IMP
    signal transduction GO:0007165 IEA
    positive regulation of cytosolic calcium ion concentration GO:0007204 ISO
    neutrophil chemotaxis GO:0030593 IMP
    defense response to Gram-positive bacterium GO:0050830 IMP
    positive regulation of ERK1 and ERK2 cascade GO:0070374 ISO
    positive regulation of neutrophil chemotaxis GO:0090023 IMP
    complement component C5a signaling pathway GO:0038178 ISO
    apoptotic process GO:0006915 ISO
    chemotaxis GO:0006935 IEA
    negative regulation of neuron apoptotic process GO:0043524 ISO
Subcellular Localization
    basolateral plasma membrane GO:0016323 ISO
    membrane GO:0016020 IEA
    cytosol GO:0005829 ISO
    cell surface GO:0009986 ISO
    plasma membrane GO:0005886 ISO
    cytoplasmic vesicle GO:0031410 IEA
    apical part of cell GO:0045177 ISO
    integral component of membrane GO:0016021 IEA
 Experiment description of studies that identified C5ar1 in exosomes
1
Experiment ID 214
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for C5ar1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which C5ar1 is involved
No pathways found





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