Gene description for Klf4
Gene name Kruppel-like factor 4 (gut)
Gene symbol Klf4
Other names/aliases EZF
Species Mus musculus
 Database cross references - Klf4
ExoCarta ExoCarta_16600
Entrez Gene 16600
UniProt Q60793  
 Klf4 identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Klf4
Molecular Function
    sequence-specific DNA binding GO:0043565 ISO
    sequence-specific DNA binding transcription factor recruiting transcription factor activity GO:0001010 IDA
    core promoter proximal region sequence-specific DNA binding GO:0000987 ISO
    metal ion binding GO:0046872 IEA
    RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription GO:0001077 ISO
    histone deacetylase binding GO:0042826 ISO
    RNA polymerase II transcription factor binding GO:0001085 ISO
    phosphatidylinositol 3-kinase regulator activity GO:0035014 ISO
    beta-catenin binding GO:0008013 IPI
    sequence-specific DNA binding transcription factor activity GO:0003700 ISO
    DNA binding GO:0003677 ISS
    transcription regulatory region DNA binding GO:0044212 ISO
    core promoter binding GO:0001047 IDA
    transcription cofactor binding GO:0001221 ISO
    transcription factor binding GO:0008134 IPI
    nucleic acid binding GO:0003676 IEA
    RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription GO:0001190 ISO
    double-stranded DNA binding GO:0003690 ISO
Biological Process
    negative regulation of chemokine (C-X-C motif) ligand 2 production GO:2000342 ISO
    regulation of transcription, DNA-templated GO:0006355 IEA
    transcription from RNA polymerase II promoter GO:0006366 ISO
    negative regulation of NF-kappaB transcription factor activity GO:0032088 ISO
    response to organic substance GO:0010033 IDA
    negative regulation of interleukin-8 biosynthetic process GO:0045415 ISO
    fat cell differentiation GO:0045444 IMP
    negative regulation of heterotypic cell-cell adhesion GO:0034115 ISO
    negative regulation of protein kinase B signaling GO:0051898 ISO
    positive regulation of transcription, DNA-templated GO:0045893 IDA
    positive regulation of telomerase activity GO:0051973 ISO
    negative regulation of ERK1 and ERK2 cascade GO:0070373 ISO
    transcription, DNA-templated GO:0006351 IEA
    cellular response to laminar fluid shear stress GO:0071499 ISO
    post-embryonic camera-type eye development GO:0031077 IMP
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 ISO
    cell differentiation GO:0030154 IMP
    negative regulation of cell proliferation GO:0008285 IDA
    response to retinoic acid GO:0032526 IDA
    negative regulation of transcription, DNA-templated GO:0045892 NAS
    post-embryonic hemopoiesis GO:0035166 ISO
    epidermis morphogenesis GO:0048730 IMP
    positive regulation of hemoglobin biosynthetic process GO:0046985 ISO
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISO
    negative regulation of smooth muscle cell proliferation GO:0048662 ISO
    negative regulation of muscle hyperplasia GO:0014740 ISO
    negative regulation of phosphatidylinositol 3-kinase signaling GO:0014067 ISO
    negative regulation of cell migration involved in sprouting angiogenesis GO:0090051 ISO
    regulation of transcription from RNA polymerase II promoter GO:0006357 IDA
    negative regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043154 ISO
    stem cell maintenance GO:0019827 IDA
    cellular response to growth factor stimulus GO:0071363 ISO
    epidermal cell differentiation GO:0009913 IMP
    positive regulation of protein metabolic process GO:0051247 ISO
    regulation of phosphatidylinositol 3-kinase activity GO:0043551 ISO
    positive regulation of nitric oxide biosynthetic process GO:0045429 ISO
    negative regulation of response to cytokine stimulus GO:0060761 ISO
    positive regulation of cellular protein metabolic process GO:0032270 ISO
    canonical Wnt signaling pathway GO:0060070 IMP
    regulation of cell differentiation GO:0045595 IDA
    regulation of cell proliferation GO:0042127 IGI
Subcellular Localization
    nuclear chromatin GO:0000790 ISO
    nuclear euchromatin GO:0005719 IDA
    nucleus GO:0005634 NAS
    chromatin GO:0000785 ISO
    transcription factor complex GO:0005667 ISO
    nucleoplasm GO:0005654 ISO
    nuclear transcription factor complex GO:0044798 IDA
 Experiment description of studies that identified Klf4 in exosomes
Experiment ID 15
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
Isolation/purification methods Filtration
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
 Protein-protein interactions for Klf4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.

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