Gene description for Akap5
Gene name A kinase (PRKA) anchor protein 5
Gene symbol Akap5
Other names/aliases AKAP150
Akap79
P150
Species Rattus norvegicus
 Database cross references - Akap5
ExoCarta ExoCarta_171026
Entrez Gene 171026
 Akap5 identified in exosomes derived from the following tissue/cell type
Pancreatic cancer cells 20124479    
Pancreatic cancer cells 20124479    
 Gene ontology annotations for Akap5
Molecular Function
    protein domain specific binding GO:0019904 IPI
    protein kinase A regulatory subunit binding GO:0034237 IDA
    adenylate cyclase binding GO:0008179 ISO
    kinase binding GO:0019900 IDA
    G-protein coupled receptor binding GO:0001664 IPI
    protein complex binding GO:0032403 IPI
    protein kinase binding GO:0019901 IPI
    calmodulin binding GO:0005516 IEA
    cadherin binding GO:0045296 IPI
    protein binding GO:0005515 IPI
    GABA receptor binding GO:0050811 IDA
    actin binding GO:0003779 IDA
    protein phosphatase 2B binding GO:0030346 IDA
Biological Process
    positive regulation of protein kinase A signaling GO:0010739 ISO
    negative regulation of adenylate cyclase activity GO:0007194 IDA
    cellular response to organonitrogen compound GO:0071417 IEP
    protein targeting GO:0006605 IEA
    positive regulation of protein phosphorylation GO:0001934 IMP
    maternal process involved in female pregnancy GO:0060135 IEP
    negative regulation of ion transport GO:0043271 IMP
    spinal cord development GO:0021510 IEP
    signal transduction GO:0007165 IEA
    negative regulation of potassium ion transport GO:0043267 IMP
    positive regulation of dendrite morphogenesis GO:0050775 IMP
    cellular protein complex disassembly GO:0043624 IDA
    amylase secretion GO:0036394 ISO
    response to electrical stimulus GO:0051602 IEP
    response to muscle activity GO:0014850 IEP
    hippocampus development GO:0021766 IEP
    positive regulation of adenylate cyclase activity GO:0045762 IDA
    positive regulation of cAMP biosynthetic process GO:0030819 ISO
    positive regulation of NFAT protein import into nucleus GO:0051533 IMP
    positive regulation of calcium ion transport into cytosol GO:0010524 IDA
Subcellular Localization
    perinuclear region of cytoplasm GO:0048471 IDA
    dendritic spine membrane GO:0032591 IDA
    protein complex GO:0043234 IDA
    cytoplasm GO:0005737 IDA
    dendritic shaft GO:0043198 IDA
    basolateral plasma membrane GO:0016323 IDA
    filopodium membrane GO:0031527 IDA
    dendritic spine GO:0043197 IDA
    plasma membrane GO:0005886 TAS
    asymmetric synapse GO:0032279 IDA
    cytoskeleton GO:0005856 ISO
    dendrite membrane GO:0032590 IDA
    postsynaptic density GO:0014069 IDA
    cell GO:0005623 ISO
    neuronal cell body GO:0043025 IDA
 Experiment description of studies that identified Akap5 in exosomes
1
Experiment ID 92
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|GAPDH
EV Cytosolic markers
✔
CD9|CD151
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
RatRef-12 Microarray
FACS
Mass spectrometry [MALDI TOF]
2
Experiment ID 93
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|GAPDH
EV Cytosolic markers
✔
CD9|CD151
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
 Protein-protein interactions for Akap5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Adrb2  
Affinity Capture-Western Rattus norvegicus
Reconstituted Complex Rattus norvegicus
View the network image/svg+xml
 Pathways in which Akap5 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here