Gene description for Prkar1a
Gene name protein kinase, cAMP dependent regulatory, type I, alpha
Gene symbol Prkar1a
Other names/aliases 1300018C22Rik
RIalpha
Tse-1
Tse1
Species Mus musculus
 Database cross references - Prkar1a
ExoCarta ExoCarta_19084
Entrez Gene 19084
UniProt Q9DBC7  
 Prkar1a identified in exosomes derived from the following tissue/cell type
Mast cells 17486113    
 Gene ontology annotations for Prkar1a
Molecular Function
    ubiquitin protein ligase binding GO:0031625 ISO
    nucleotide binding GO:0000166 IEA
    cAMP binding GO:0030552 ISO
    protein kinase A catalytic subunit binding GO:0034236 ISO
    cAMP-dependent protein kinase inhibitor activity GO:0004862 ISO
    protein binding GO:0005515 IPI
    cAMP-dependent protein kinase regulator activity GO:0008603 ISO
Biological Process
    organ morphogenesis GO:0009887 TAS
    cardiac muscle cell proliferation GO:0060038 IMP
    negative regulation of cAMP-dependent protein kinase activity GO:2000480 ISO
    cell proliferation GO:0008283 TAS
    heart development GO:0007507 IMP
    protein phosphorylation GO:0006468 TAS
    sarcomere organization GO:0045214 IMP
    negative regulation of protein kinase activity GO:0006469 IMP
    mesoderm formation GO:0001707 IMP
    regulation of protein phosphorylation GO:0001932 IEA
    regulation of protein kinase activity GO:0045859 ISO
Subcellular Localization
    neuromuscular junction GO:0031594 IDA
    AMP-activated protein kinase complex GO:0031588 ISO
    membrane GO:0016020 ISO
    cAMP-dependent protein kinase complex GO:0005952 ISO
    plasma membrane GO:0005886 IEA
    protein complex GO:0043234 ISO
    cytoplasm GO:0005737 TAS
 Experiment description of studies that identified Prkar1a in exosomes
1
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
 Protein-protein interactions for Prkar1a
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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