Gene description for Stk10
Gene name serine/threonine kinase 10
Gene symbol Stk10
Other names/aliases Gek1
Lok
mKIAA4026
Species Mus musculus
 Database cross references - Stk10
ExoCarta ExoCarta_20868
Entrez Gene 20868
UniProt O55098  
 Stk10 identified in exosomes derived from the following tissue/cell type
Macrophages 23658846    
Macrophages 23658846    
Macrophages 23658846    
 Gene ontology annotations for Stk10
Molecular Function
    identical protein binding GO:0042802 ISO
    protein homodimerization activity GO:0042803 ISO
    transferase activity, transferring phosphorus-containing groups GO:0016772 IEA
    transferase activity GO:0016740 IEA
    nucleotide binding GO:0000166 IEA
    protein serine/threonine kinase activity GO:0004674 TAS
    kinase activity GO:0016301 IEA
    protein kinase activity GO:0004672 IEA
    ATP binding GO:0005524 IEA
Biological Process
    cell cycle GO:0007049 IEA
    phosphorylation GO:0016310 IEA
    activation of protein kinase activity GO:0032147 IBA
    protein phosphorylation GO:0006468 TAS
    lymphocyte aggregation GO:0071593 IMP
    signal transduction by protein phosphorylation GO:0023014 IBA
    regulation of mitotic cell cycle GO:0007346 IBA
    regulation of apoptotic process GO:0042981 IBA
    regulation of lymphocyte migration GO:2000401 ISO
    stress-activated protein kinase signaling cascade GO:0031098 IBA
    protein autophosphorylation GO:0046777 ISO
Subcellular Localization
    membrane GO:0016020 IEA
    extracellular exosome GO:0070062 ISO
    plasma membrane GO:0005886 ISO
    cytoplasm GO:0005737 IBA
 Experiment description of studies that identified Stk10 in exosomes
1
Experiment ID 214
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Leishmania-infected-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 215
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name LPS-treated-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 216
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23658846    
Organism Mus musculus
Experiment description Immunomodulatory impact of leishmania-induced macrophage exosomes: a comparative proteomic and functional analysis.
Authors Hassani K, Olivier M.
Journal name PLoS Negl Trop Dis
Publication year 2013
Sample Macrophages
Sample name Normal-Macrophage (J774A.1)
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Filtration
Protease inhibitors
Sucrose density gradient
Filtration
Ultracentrifugation
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Stk10
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Stk10 is involved
No pathways found





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