Gene description for Gdi1
Gene name GDP dissociation inhibitor 1
Gene symbol Gdi1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Gdi1
ExoCarta ExoCarta_25183
Entrez Gene 25183
UniProt P50398  
 Gdi1 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Gdi1
Molecular Function
    GTPase activator activity GO:0005096 IEA
    Rab GTPase binding GO:0017137 IPI
    protein binding GO:0005515 IPI
    Rab GDP-dissociation inhibitor activity GO:0005093 IDA
Biological Process
    negative regulation of protein targeting to membrane GO:0090315 ISS
    Rab protein signal transduction GO:0032482 ISS
    positive regulation of GTPase activity GO:0043547 IEA
    regulation of catalytic activity GO:0050790 IDA
    negative regulation of axonogenesis GO:0050771 IDA
    protein transport GO:0015031 IEA
    response to calcium ion GO:0051592 IDA
Subcellular Localization
    cytoplasm GO:0005737 ISO
    myelin sheath GO:0043209 ISO
    midbody GO:0030496 ISO
    protein complex GO:0043234 IDA
    neuron projection GO:0043005 IDA
    Golgi apparatus GO:0005794 IEA
 Experiment description of studies that identified Gdi1 in exosomes
1
Experiment ID 95
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 101
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Gdi1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Gdi1 is involved
PathwayEvidenceSource
Rho GTPase cycle IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here