Gene description for Acsl1
Gene name acyl-CoA synthetase long-chain family member 1
Gene symbol Acsl1
Other names/aliases ACS
Acas
COAA
Facl2
Species Rattus norvegicus
 Database cross references - Acsl1
ExoCarta ExoCarta_25288
Entrez Gene 25288
UniProt P18163  
 Acsl1 identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
Hepatocytes 19367702    
Reticulocytes 21828046    
 Gene ontology annotations for Acsl1
Molecular Function
    acetate-CoA ligase activity GO:0003987 TAS
    ATP binding GO:0005524 IEA
    long-chain fatty acid-CoA ligase activity GO:0004467 ISO
Biological Process
    fatty acid transport GO:0015908 IGI
    long-chain fatty acid metabolic process GO:0001676 ISO
    xenobiotic catabolic process GO:0042178 IDA
    adiponectin-activated signaling pathway GO:0033211 ISO
    long-chain fatty acid import GO:0044539 ISO
    lipid metabolic process GO:0006629 TAS
    triglyceride metabolic process GO:0006641 IGI
    response to organic cyclic compound GO:0014070 IEP
    response to oleic acid GO:0034201 IEP
    positive regulation of protein serine/threonine kinase activity GO:0071902 ISO
    response to drug GO:0042493 IEP
    response to organic substance GO:0010033 IDA
    fatty acid metabolic process GO:0006631 IMP
    response to nutrient GO:0007584 IEP
    lipid biosynthetic process GO:0008610 ISO
Subcellular Localization
    plasma membrane GO:0005886 IDA
    mitochondrial outer membrane GO:0005741 IDA
    integral component of membrane GO:0016021 IEA
    mitochondrion GO:0005739 ISO
    endoplasmic reticulum GO:0005783 IDA
    membrane GO:0016020 ISO
    peroxisomal membrane GO:0005778 IDA
    endoplasmic reticulum membrane GO:0005789 IEA
 Experiment description of studies that identified Acsl1 in exosomes
1
Experiment ID 225
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 35
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
CD81|CD63
EV Membrane markers
HSPA5
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
3
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Acsl1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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