Gene description for Vcam1
Gene name vascular cell adhesion molecule 1
Gene symbol Vcam1
Other names/aliases VCAM1B
Species Rattus norvegicus
 Database cross references - Vcam1
ExoCarta ExoCarta_25361
Entrez Gene 25361
UniProt P29534  
 Vcam1 identified in exosomes derived from the following tissue/cell type
Pancreatic cancer cells 20124479    
Pancreatic cancer cells 20124479    
 Gene ontology annotations for Vcam1
Molecular Function
    integrin binding GO:0005178 ISO
    cell adhesion molecule binding GO:0050839 ISO
    primary amine oxidase activity GO:0008131 ISO
Biological Process
    response to nicotine GO:0035094 IEP
    cellular response to vascular endothelial growth factor stimulus GO:0035924 IEP
    response to nutrient GO:0007584 IEP
    response to hypoxia GO:0001666 IEP
    membrane to membrane docking GO:0022614 ISO
    response to lipopolysaccharide GO:0032496 IEP
    leukocyte tethering or rolling GO:0050901 ISO
    heart development GO:0007507 ISO
    chronic inflammatory response GO:0002544 IEP
    aging GO:0007568 IEP
    viral process GO:0016032 IEA
    acute inflammatory response GO:0002526 IEP
    cellular response to glucose stimulus GO:0071333 ISO
    embryonic placenta morphogenesis GO:0060669 ISO
    amine metabolic process GO:0009308 ISO
    cell adhesion GO:0007155 TAS
    oxidation-reduction process GO:0055114 ISO
    heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules GO:0007157 ISO
    leukocyte cell-cell adhesion GO:0007159 ISO
    chorio-allantoic fusion GO:0060710 ISO
    response to ethanol GO:0045471 IEP
    cell chemotaxis GO:0060326 IMP
    response to zinc ion GO:0010043 IEP
    positive regulation of T cell proliferation GO:0042102 ISO
    cell-matrix adhesion GO:0007160 ISO
    response to ionizing radiation GO:0010212 IEP
    cellular response to tumor necrosis factor GO:0071356 IEP
Subcellular Localization
    cell periphery GO:0071944 ISO
    endoplasmic reticulum GO:0005783 ISO
    Golgi apparatus GO:0005794 ISO
    early endosome GO:0005769 ISO
    cell surface GO:0009986 ISO
    alpha9-beta1 integrin-vascular cell adhesion molecule-1 complex GO:0071065 ISO
    podosome GO:0002102 ISO
    filopodium GO:0030175 ISO
    sarcolemma GO:0042383 IDA
    integral component of membrane GO:0016021 TAS
    microvillus GO:0005902 ISO
    apical part of cell GO:0045177 ISO
    extracellular exosome GO:0070062 ISO
    external side of plasma membrane GO:0009897 ISO
    extracellular space GO:0005615 ISO
 Experiment description of studies that identified Vcam1 in exosomes
1
Experiment ID 93
ISEV standards
EM
EV Biophysical techniques
HSP70|GAPDH
EV Cytosolic markers
CD9|CD151
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [MALDI TOF]
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
2
Experiment ID 92
ISEV standards
EM
EV Biophysical techniques
HSP70|GAPDH
EV Cytosolic markers
CD9|CD151
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
RatRef-12 Microarray
FACS
Mass spectrometry [MALDI TOF]
3
Experiment ID 93
ISEV standards
EM
EV Biophysical techniques
HSP70|GAPDH
EV Cytosolic markers
CD9|CD151
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
 Protein-protein interactions for Vcam1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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